Sulistina Dewi Ratna, Martini Santi
Doctoral Program of Public Health.
Department of Epidemiology, Faculty of Public Health, Universitas Airlangga, Mulyorejo, Surabaya, Indonesia.
J Public Health Res. 2020 Jul 2;9(2):1812. doi: 10.4081/jphr.2020.1812. eCollection 2020 Jul 3.
Rhodamine B is a component of xenobiotic substance metabolized by cytochrome P450 in the body to produce free radicals, which affects the activity of Superoxidase Dismutase (SOD), thereby, leading to oxidative stress, injury, increase in cell apoptosis and brainstem. This study aims to determine the effect of Rhodamine B on BAX and BCL-2 in the cerebellum and brainstem tissue of . The True Experimental Design was used to carry out a post-test examination on the control group of twenty-eight Wistar female mice between the ages of 10-12 weeks. Then, samples were categorized into 4 groups in body weight doses of 4.5 mg/200g, 9 mg/200g, and 18 mg/200g. They were administrated with Rhodamine B personde for 36 days. The results showed that Rhodamine B had a direct and indirect effect on BAX and BCL-2 expressions, respectively, in the cerebellum tissue and wistar strain of . In addition, the positive path coefficient of BAX expression has a positive effect on BCL-2. This means that an increase in BAX has a direct impact on decreasing BCL-2 expression in cerebellum tissue and brainstem of wistar strain along with an increased dose of Rhodamine B. In conclusion, Rhodamine B tends to increase BAX expression which directly decreases BCL-2 in Cerebellum tissue and Brainstem in along with increasing doses.
罗丹明B是一种外源性物质的成分，在体内被细胞色素P450代谢产生自由基，影响超氧化物歧化酶（SOD）的活性，从而导致氧化应激、损伤、细胞凋亡增加以及脑干病变。本研究旨在确定罗丹明B对[具体对象]小脑和脑干组织中BAX和BCL-2的影响。采用真实验设计对28只10 - 12周龄的Wistar雌性小鼠对照组进行后测检查。然后，将样本按体重剂量分为4.5 mg/200g、9 mg/200g和18 mg/200g三组。给它们腹腔注射罗丹明B 36天。结果表明，罗丹明B分别对[具体对象]小脑组织和Wistar品系中的BAX和BCL-2表达有直接和间接影响。此外，BAX表达的正向路径系数对BCL-2有正向影响。这意味着随着罗丹明B剂量增加，BAX增加对[具体对象]小脑组织和脑干中BCL-2表达降低有直接影响。总之，随着剂量增加，罗丹明B倾向于增加[具体对象]小脑组织和脑干中BAX的表达，从而直接降低BCL-2的表达。
