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补充 9-视网膜可加速人多能干细胞来源的视网膜类器官中 Rod 光感受器的发育。

Accelerated Development of Rod Photoreceptors in Retinal Organoids Derived from Human Pluripotent Stem Cells by Supplementation with 9- Retinal.

机构信息

Neurobiology, Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.

Technical Contact.

出版信息

STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2020.100033. Epub 2020 Jun 3.

DOI:10.1016/j.xpro.2020.100033
PMID:32728670
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7388645/
Abstract

Human pluripotent stem cells (PSCs) can be differentiated into retinal organoids with proper neural layer organization, yet the protocols are technically challenging and time consuming. We have modified a widely used differentiation protocol by switching all- retinoic acid with 9- retinal to accelerate photoreceptor differentiation and improve morphogenesis. In this report, we provide a detailed and improved protocol to generate retinal organoids from human pluripotent stem cells. For complete details on the use and execution of this protocol, please refer to Kaya et al. (2019).

摘要

人多能干细胞（PSCs）可以分化为具有适当神经层组织的视网膜类器官，但该技术具有挑战性，且耗时。我们通过将全视黄酸替换为 9-视黄醛来加速光感受器分化并改善形态发生，修改了一个广泛使用的分化方案。在本报告中，我们提供了一个从人多能干细胞生成视网膜类器官的详细改进方案。有关该方案使用和执行的完整详细信息，请参阅 Kaya 等人。（2019 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/4c1503cfb64a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/c43aaeadab3b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/5628341131d4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/e5fc64cd7d64/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/4c1503cfb64a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/c43aaeadab3b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/5628341131d4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/e5fc64cd7d64/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ddc/7580118/4c1503cfb64a/gr3.jpg

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Mol Vis. 2020 Mar 3;26:97-105. eCollection 2020.
2
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Mol Vis. 2019 Nov 11;25:663-678. eCollection 2019.
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Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs.
Retinal Organoids: Innovative Tools for Understanding Retinal Degeneration.
视网膜类器官：理解视网膜变性的创新工具。
Int J Mol Sci. 2025 Apr 1;26(7):3263. doi: 10.3390/ijms26073263.
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Generation of Retinal Organoids Using Human-Induced Pluripotent Stem Cells.利用人类诱导多能干细胞生成视网膜类器官
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Front Transplant. 2023 Sep 29;2:1130086. doi: 10.3389/frtra.2023.1130086. eCollection 2023.
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Retinoid Synthesis Regulation by Retinal Cells in Health and Disease.视网膜细胞在健康和疾病中的类维生素 A 合成调控。
Cells. 2024 May 18;13(10):871. doi: 10.3390/cells13100871.
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