Department of Neurosurgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Science, Third Hospital of Shanxi Medical University, Shanxi, China.
Department of Otolaryngology Head and Neck Surgery, First Hospital of Shanxi Medical University, Shanxi, China.
Folia Neuropathol. 2020;58(2):176-193. doi: 10.5114/fn.2020.96983.
Stroke is partial or complete brain dysfunction combined with acute cerebral circulatory disorders, and it affects millions of individuals around the world each year. A total of 70-80% of patients experience ischaemic stroke caused by disturbances in cerebral circulation, leading to cerebral ischaemia, neuronal apoptosis, and necrosis. Tanshinone IIA is a natural compound extracted from Salvia miltiorrhiza and has been proven to assist in recovery from cerebral ischaemia reperfusion injury. GLUT1 is ubiquitously expressed in all types of tissues in the human body and has important physiological functions due to its glucose uptake ability. This experiment was performed to detect the effect of GLUT1 in promoting the therapeutic effect of tanshinone IIA. Here, we found that tanshinone IIA treatment increased the viability of neurons and promoted the recovery of brain function, and that the concentration of glucose in serum and cultured medium was also increased. We noticed that these effects might be mediated by an increased glucose uptake ability. In addition, we further found that the PI3K/mTOR/HER3 signalling pathway played an important role in regulating these effects. Thus, we thought that overexpression of GLUT1 might be an important target in the treatment of cerebral ischaemia-reperfusion.
中风是一种局部或完全的大脑功能障碍，伴有急性脑循环紊乱，每年影响全球数百万人。大约 70-80%的患者因脑循环紊乱而发生缺血性中风，导致脑缺血、神经元凋亡和坏死。丹参酮 IIA 是一种从丹参中提取的天然化合物，已被证明有助于恢复脑缺血再灌注损伤。GLUT1 在人体所有类型的组织中广泛表达，由于其摄取葡萄糖的能力，具有重要的生理功能。本实验旨在检测 GLUT1 在促进丹参酮 IIA 治疗效果中的作用。在这里，我们发现丹参酮 IIA 治疗可增加神经元的活力并促进脑功能的恢复，同时血清和培养基中的葡萄糖浓度也增加了。我们注意到这些作用可能是通过增加葡萄糖摄取能力来介导的。此外，我们还进一步发现 PI3K/mTOR/HER3 信号通路在调节这些作用中起着重要作用。因此，我们认为过表达 GLUT1 可能是治疗脑缺血再灌注的一个重要靶点。
