Department of Chemistry, Illinois Institute of Technology, 3101 S Dearborn St, Chicago, IL, 60616, USA.
Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA.
Biosens Bioelectron. 2020 Oct 1;165:112289. doi: 10.1016/j.bios.2020.112289. Epub 2020 May 16.
Proteins are essential for all living organisms, and perform a wide variety of functions in the cell and human body, including structural, mechanical, biochemical, and signaling. Since proteins can serve as valuable biomarkers for health status and diseases states, and enable personalized medicine, sensitive and rapid detection of proteins is of paramount importance. Herein, we report a chemically functionalized conical shaped poly-(ethylene terephthalate) nanopore (PET nanopore) as a stochastic sensing element for detection of proteins at the single-molecule level. We demonstrate that the PET nanopore sensor is not only sensitive and selective, but also can differentiate proteins rapidly, offering the potential for label-free protein detection and characterization. Our developed PET nanopore sensing strategy not only provides a general platform for exploring fundamental protein dynamics and rapid detection of proteins at the single-molecule level, but also opens new avenues toward advanced deeper understanding of enzymes, development of more efficient biosensing technologies, and constructing novel biomimetic nanopore systems.
蛋白质是所有生物的基本组成部分，在细胞和人体中执行着广泛的功能，包括结构、机械、生化和信号传递。由于蛋白质可以作为健康状况和疾病状态的有价值的生物标志物，并实现个性化医疗，因此敏感和快速的蛋白质检测至关重要。在此，我们报告了一种化学功能化的锥形聚对苯二甲酸乙二醇酯（PET）纳米孔（PET 纳米孔）作为一种随机传感元件，用于在单分子水平上检测蛋白质。我们证明，PET 纳米孔传感器不仅具有灵敏度和选择性，而且能够快速区分蛋白质，为无标记蛋白质检测和表征提供了可能性。我们开发的 PET 纳米孔传感策略不仅为探索基本蛋白质动力学和单分子水平上的快速蛋白质检测提供了一个通用平台，而且为深入了解酶、开发更高效的生物传感技术以及构建新型仿生纳米孔系统开辟了新的途径。
