Department of Chemistry, Illinois Institute of Technology, 3101 S Dearborn St, Chicago, IL 60616, USA.
Nanoscale. 2021 Aug 28;13(32):13658-13664. doi: 10.1039/d1nr04085e. Epub 2021 Aug 3.
Multiplexing methods which are capable of measurement of multiple analytes in a single assay are of great importance in many fields. The conventional strategy for simultaneous detection of multiple species is to construct a sensor array. Herein, we report an innovative multiplex multi-analyte detection platform in a non-array format for protease measurement. By monitoring protease degradation of a single peptide substrate containing two cleavage sites for a disintegrin and metalloproteinase 10 (ADAM10) and a disintegrin and metalloproteinase 10 (ADAM17) in a single nanopore, simultaneous detection and quantification of these two model proteases in mixture samples could satisfactorily be accomplished. Our developed multiplexing sensing platform has the potential to be coupled with the traditional sensor array to further improve the multiplexing capability of the sensor, which may find useful applications in clinical diagnosis and prognosis.
在许多领域中，能够在单次分析中测量多种分析物的多重分析方法非常重要。传统的同时检测多种物质的策略是构建传感器阵列。在此，我们报告了一种用于蛋白酶测量的非阵列格式的创新型多重多分析物检测平台。通过在单个纳米孔中监测包含两个用于解整合素金属蛋白酶 10 (ADAM10) 和解整合素金属蛋白酶 17 (ADAM17) 的切割位点的单一肽底物的蛋白酶降解，可令人满意地完成混合物样品中这两种模型蛋白酶的同时检测和定量。我们开发的多重传感平台有可能与传统的传感器阵列相结合，以进一步提高传感器的多重分析能力，这可能在临床诊断和预后中有实用的应用。
