Ford Suzanne A, Albert Istvan, Allen Scott L, Chenoweth Stephen F, Jones Matthew, Koh Cassandra, Sebastian Aswathy, Sigle Leah T, McGraw Elizabeth A
Huck Institute of Life Sciences, Penn State University, University Park, PA, United States.
School of Biological Sciences, Monash University, Melbourne, VIC, Australia.
Front Microbiol. 2020 Jul 7;11:1456. doi: 10.3389/fmicb.2020.01456. eCollection 2020.
is an intracellular bacterium that blocks virus replication in insects and has been introduced into the mosquito, for the biocontrol of arboviruses including dengue, Zika, and chikungunya. Despite ongoing research, the mechanism of mediated virus blocking remains unclear. We recently used experimental evolution to reveal that mediated dengue blocking could be selected upon in the host and showed evidence that strong levels of blocking could be maintained by natural selection. In this study, we investigate the genetic variation associated with blocking and use these analyses to generate testable hypotheses surrounding the mechanism of mediated dengue blocking. From our results, we hypothesize that may block virus replication by increasing the regeneration rate of mosquito cells via the Notch signaling pathway. We also propose that modulates the host's transcriptional pausing pathway either to prime the host's anti-viral response or to directly inhibit viral replication.
是一种细胞内细菌，可阻断昆虫体内的病毒复制，已被引入蚊子体内，用于生物防治包括登革热、寨卡病毒和基孔肯雅热在内的虫媒病毒。尽管研究仍在进行，但介导的病毒阻断机制仍不清楚。我们最近利用实验进化揭示，介导的登革热阻断可以在宿主中被选择，并表明有证据表明高水平的阻断可以通过自然选择得以维持。在本研究中，我们调查了与阻断相关的基因变异，并利用这些分析来生成围绕介导的登革热阻断机制的可测试假设。根据我们的结果，我们假设可能通过Notch信号通路提高蚊子细胞的再生率来阻断病毒复制。我们还提出，调节宿主的转录暂停途径，要么启动宿主的抗病毒反应，要么直接抑制病毒复制。
