对受污染的MGIT 960培养物进行再处理以提高分枝杆菌有效结果的可得性。
Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria.
作者信息
Subramanyam Balaji, Sivaramakrishnan Gomathi, Sangamithrai Devi, Ravi Rajkumar, Thiruvengadam Kannan, Vijayaragavan Vaishnavi, Selvaraju Ayswarya, Tripathy Srikanth Prasad, Mondal Rajesh
机构信息
Department of Bacteriology, ICMR-National Institute for Research in Tuberculosis, Chennai, India.
出版信息
Int J Microbiol. 2020 Jul 18;2020:1721020. doi: 10.1155/2020/1721020. eCollection 2020.
Optimal recovery of mycobacteria from the contaminated liquid culture is a challenge. While alternative methods have been suggested to reduce the rate of contamination in the BACTEC MGIT 960 system, reprocessing the contaminated liquid culture improves recovery of . Among 793 MGIT cultures raised from as many sputum specimens after primary decontamination by the standard NaLC-NaOH method, valid results were available for 687 (86.6%) as 106 (13.4%) were contaminated. Reprocessing and reculturing of the contaminated cultures increased valid results to 739 (93.2%) and reduced the contamination rate to 6.8%. Both values were statistically significant. Recovery of the complex increased from 45.6% to 48.4%. Valid negative results were available for an additional 3.4%. The method may be adopted to reduce the rate of contamination and to improve the valid culture results for mycobacteria.
从受污染的液体培养物中最佳地回收分枝杆菌是一项挑战。虽然已提出替代方法来降低BACTEC MGIT 960系统中的污染率，但对受污染的液体培养物进行再处理可提高回收效果。在用标准的NaLC-NaOH方法进行初次去污后，从793份痰液标本中培养出MGIT培养物，其中687份（86.6%）获得了有效结果，106份（13.4%）受到污染。对受污染的培养物进行再处理和重新培养后，有效结果增加到739份（93.2%），污染率降低到6.8%。这两个值均具有统计学意义。分枝杆菌复合物的回收率从45.6%提高到48.4%。另外还有3.4%获得了有效的阴性结果。该方法可用于降低污染率并改善分枝杆菌的有效培养结果。
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