Thieme Susann, Hafez Hafez M, Gutzer Stefanie, Warkentin Nadine, Lüschow Dörte, Mühldorfer Kristin
Institute of Poultry Diseases, Freie Universität Berlin, Königsweg 63, 14163 Berlin, Germany.
Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.
Vet Anim Sci. 2016 Oct 17;1-2:15-20. doi: 10.1016/j.vas.2016.10.002. eCollection 2016 Dec.
The sudden emergence of (ORT) in commercially raised poultry species and its presence in non-galliform birds raise important epidemiological issues about the role of interspecies transmission. In the present study, 21 ORT strains isolated from pigeons and from birds of prey were analyzed using the recently established multilocus sequence typing (MLST) scheme. Results were compared to MLST sequence data available from ORT strains isolated mainly from turkeys and chickens, but also single strains from pheasant, guineafowl and rook. The pigeon-derived ORT strains (n=11) were closely related amongst themselves representing their own cluster distant from ORT strains of non-columbiform avian hosts. ORT strains isolated from birds of prey (n=10) revealed a higher genetic heterogeneity that corresponded well to their host family relationships but grouped within the two mainly poultry-based clusters. None of these strains had a sequence type identical to strains investigated previously. However, three strains isolated from common kestrels and a single strain from a turkey vulture shared one or two out of seven gene loci, respectively, with strains of turkey and chicken origin. The MLST results of ORT isolated from pigeons and birds of prey likely reflect evolutionary bacterial host adaptations but might also indicate a potential for interspecies transmission. Definite conclusions should be drawn carefully as so far a few strains from non-galliform birds were analyzed by MLST. By extending the number of ORT isolates and the range of potential avian hosts, the MLST database can provide a valuable resource in understanding transmission dynamics.
商业养殖家禽品种中突然出现禽网状内皮组织增殖症（ORT），且在非鸡形目鸟类中也有发现，这引发了关于种间传播作用的重要流行病学问题。在本研究中，使用最近建立的多位点序列分型（MLST）方案对从鸽子和猛禽中分离出的21株ORT菌株进行了分析。将结果与主要从火鸡和鸡中分离出的ORT菌株，以及从雉鸡、珍珠鸡和白嘴鸦中分离出的单株菌株的MLST序列数据进行了比较。源自鸽子的ORT菌株（n = 11）彼此密切相关，形成了与非鸽形目鸟类宿主的ORT菌株不同的独立簇。从猛禽中分离出的ORT菌株（n = 10）显示出更高的遗传异质性，这与它们的宿主家族关系非常吻合，但归入了两个主要以家禽为基础的簇中。这些菌株中没有一个的序列类型与先前研究的菌株相同。然而，从普通红隼中分离出的三株菌株和从土耳其兀鹰中分离出的一株菌株分别与源自火鸡和鸡的菌株在七个基因座中的一个或两个上具有相同序列。从鸽子和猛禽中分离出的ORT的MLST结果可能反映了细菌对宿主的进化适应，但也可能表明存在种间传播的可能性。由于到目前为止通过MLST分析的非鸡形目鸟类的菌株数量较少，因此应谨慎得出明确结论。通过扩大ORT分离株的数量和潜在鸟类宿主的范围，MLST数据库可为理解传播动态提供宝贵资源。
