Yousefinejad Sedigheh, Khosravi Mohammad, Gharibi Darioush, Mayahi Mansour, Shapouri Masoud Reza Seyfi Abad
Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Poult Sci. 2025 Jul;104(7):105211. doi: 10.1016/j.psj.2025.105211. Epub 2025 Apr 25.
Ornithobacterium rhinotracheale (ORT) is a gram-negative pathogen recognized as one of the causative agents of respiratory diseases in chickens and turkeys. This study aimed to design and evaluate the diagnostic validity of the latex agglutination test for ORT detection. A Western blot test was conducted to assess the various serotypes present in the bacterial isolates, as well as the immunogenicity of their lipopolysaccharide. Following the design and optimization of the ELISA and latex agglutination tests using prepared mixed cell membrane antigens from the different isolates, a serological evaluation of the infection was performed on a total of 244 turkey serum samples, both with and without respiratory symptoms. The protein profiles of the isolated ORT samples revealed equal expression of proteins with molecular weights of 75 kDa and 100 kDa. The collected sera exhibited common antibodies that reacted with antigens weighing 48 kDa, 75 kDa and 100 kDa in Western blot analysis. The sensitivity, specificity and similarity of the latex agglutination test, in comparison to ELISA, were 87.94, 90.29 and 88.93 %, respectively. The developed latex agglutination test resulted in a positive reaction at the presence of a minimum antibody concentration equal to 308.63 μg/mL. The results indicate that the latex agglutination test is effective for the rapid diagnosis and control of Ornithobacterium infection in turkeys.
禽气管鸟杆菌（ORT）是一种革兰氏阴性病原体，被认为是鸡和火鸡呼吸道疾病的病原体之一。本研究旨在设计并评估用于检测ORT的乳胶凝集试验的诊断有效性。进行了蛋白质印迹试验，以评估细菌分离株中存在的各种血清型及其脂多糖的免疫原性。在使用从不同分离株制备的混合细胞膜抗原设计和优化酶联免疫吸附测定（ELISA）和乳胶凝集试验后，对总共244份有或无呼吸道症状的火鸡血清样本进行了感染的血清学评估。分离的ORT样本的蛋白质谱显示分子量为75 kDa和100 kDa的蛋白质表达相同。在蛋白质印迹分析中，收集的血清显示出与重量为48 kDa、75 kDa和100 kDa的抗原发生反应的共同抗体。与ELISA相比，乳胶凝集试验的敏感性、特异性和相似度分别为87.94%、90.29%和88.93%。所开发的乳胶凝集试验在最低抗体浓度等于308.63μg/mL时产生阳性反应。结果表明，乳胶凝集试验对于快速诊断和控制火鸡中的鸟杆菌感染是有效的。
