Fonseca M D M, Maia J M S, Varago F C, Gern J C, Carvalho W A, Silva S R, Mosqueira V C F, Brandão H M, Guimarães A S
Departamento de Medicina Veterinária, Pós-graduação, Universidade Federal de Lavras, CEP 37200-000 Lavras, Minas Gerais, Brazil.
Pós-doutoranda, Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, Juiz de Fora, Minas Gerais, 36038-330, Brazil.
Vet Anim Sci. 2020 Jan 10;9:100089. doi: 10.1016/j.vas.2020.100089. eCollection 2020 Jun.
Infectious bovine keratoconjunctivitis (IBK) is a widespread, contagious ocular disease that affects cattle, especially dairy breeds. The disease is caused by Gram-negative bacteria mainly and its treatment consists of parenteral or topic antibiotic therapy. The topic treatment approach is used more commonly in lactating cows, to avoid milk disposal. However, treatment failures are common, because the antibiotic is removed during lacrimation. This study aimed to evaluate the susceptibility of commercial cloxacillin and evaluate the efficacy of nanostructured cloxacillin in clinical cases of IBK by . The minimum inhibitory concentration (MIC) of nanoparticle cloxacillin nanocoated, the nanoparticle without the antibiotic and the commercial cloxacillin were determined with field samples of (5) and (5). The efficiency of nanoparticles was tested in three cows naturally infected that were treated with 1.0 mL (with 0.32 mg of nanostructured cloxacillin) for the ocular route. was isolated and identified by biochemical and molecular methods before the treatment. The animals were treated every 12 h for six days. The cure was considered by the absence of clinical symptoms and bacteria after treatment. The mucoadhesive nanoparticle-based formulation promoted clinical cure with a low number of doses of antibiotics, probably due to the maintenance of the MIC in the ocular mucosa for longer due to the mucoadhesive characteristics of the nanoparticle. The results indicate that the use of nanocoated cloxacillin is possible to control infectious bovine keratoconjunctivitis.
传染性牛角膜结膜炎（IBK）是一种广泛传播的、传染性眼部疾病，影响牛群，尤其是奶牛品种。该疾病主要由革兰氏阴性菌引起，其治疗包括肠胃外或局部抗生素治疗。局部治疗方法在泌乳奶牛中更常用，以避免弃奶。然而，治疗失败很常见，因为抗生素在流泪过程中会被清除。本研究旨在评估市售氯唑西林的敏感性，并通过……评估纳米结构氯唑西林在IBK临床病例中的疗效。用（5）份……和（5）份……的现场样本测定了纳米颗粒包被的氯唑西林、不含抗生素的纳米颗粒和市售氯唑西林的最低抑菌浓度（MIC）。在三只自然感染的奶牛中测试了纳米颗粒的效果，通过眼部途径用1.0毫升（含0.32毫克纳米结构氯唑西林）进行治疗。在治疗前通过生化和分子方法分离并鉴定……。每12小时对动物进行一次治疗，持续六天。治疗后若无临床症状且无细菌，则视为治愈。基于纳米颗粒的粘膜粘附制剂以低剂量抗生素促进了临床治愈，这可能是由于纳米颗粒的粘膜粘附特性使MIC在眼粘膜中维持更长时间。结果表明，使用纳米包被的氯唑西林有可能控制传染性牛角膜结膜炎。
