Kuibagarov Marat, Abdullina Elmira, Ryskeldina Anara, Abdigulov Bolat, Amirgazin Asylulan, Shevtsov Alexandr, Angelos John A
National Center for Biotechnology, Astana, 010000, Kazakhstan.
Department of Veterinary and Agricultural Management, Shakarim University, Semey, 071412, Kazakhstan.
Vet World. 2023 Sep;16(9):1833-1839. doi: 10.14202/vetworld.2023.1833-1839. Epub 2023 Sep 14.
Infectious bovine keratoconjunctivitis (IBK) causes a significant economic loss to cattle industries in many countries, including Kazakhstan. Although is recognized as an etiologic agent of IBK, other bacterial and viral agents have been suspected to play a role in the pathogenesis of this disease. This study aimed to evaluate samples collected from the eyes of IBK-affected cattle in Eastern Kazakhstan at different stages of IBK for the presence of , , , , and Bovine Herpes Virus Type 1 (BHV-1) and to characterize gene sequence diversity from positive samples.
Individual ocular swabs (n = 168) were collected from cattle that had clinical signs of IBK during the summer of 2022 on farms in the Abay region of Kazakhstan. Eye lesion scores (1, 2, and 3) were assigned depending on the degree of ocular damage. Infectious bovine keratoconjunctivitis-associated organisms were detected using a multiplex real-time polymerase chain reaction assay. The gene was sequenced from positive samples.
and BHV-1 were not detected in any of the collected samples. was identified in the majority of samples overall, usually in mixed infection with spp. was detected in 76.2% of animals and predominated in animals with eye lesion scores 2 and 3. was detected only in association with and/or in animals with eye lesion scores 2 and 3. was found in 57.7% of animals and was always identified in association with another organism. Sequencing of the gene in 96 samples from positive samples identified five PilA groups. The majority belonged to PilA group A. However, three new PilA groups were identified and designated PilA groups N, O, and P.
The results indicate a high prevalence of and in eyes of cattle with IBK on livestock farms in Eastern Kazakhstan. Additional novel PilA groups were identified.
传染性牛角膜结膜炎（IBK）给包括哈萨克斯坦在内的许多国家的养牛业造成了重大经济损失。尽管[某种病原体，原文未明确写出]被认为是IBK的病原体，但其他细菌和病毒病原体也被怀疑在该病的发病机制中起作用。本研究旨在评估2022年夏季在哈萨克斯坦东部受IBK影响的牛眼睛在IBK不同阶段采集的样本中是否存在[相关病原体，原文未明确写出]、[相关病原体，原文未明确写出]、[相关病原体，原文未明确写出]、[相关病原体，原文未明确写出]和1型牛疱疹病毒（BHV - 1），并对来自[相关病原体，原文未明确写出]阳性样本的[某种基因，原文未明确写出]基因序列多样性进行表征。
2022年夏季，从哈萨克斯坦阿拜地区农场有IBK临床症状的牛身上采集了个体眼拭子（n = 168）。根据眼部损伤程度分配眼病变评分（1、2和3）。使用多重实时聚合酶链反应检测法检测与传染性牛角膜结膜炎相关的病原体。从[相关病原体，原文未明确写出]阳性样本中对[某种基因，原文未明确写出]基因进行测序。
在所有采集样本中均未检测到[相关病原体，原文未明确写出]和BHV - 1。总体上，在大多数样本中鉴定出了[相关病原体，原文未明确写出]，通常与[相关病原体，原文未明确写出]属混合感染。在76.2%的动物中检测到了[相关病原体，原文未明确写出]，且在眼病变评分为2和3的动物中占主导。仅在眼病变评分为2和3的动物中检测到[相关病原体，原文未明确写出]与[相关病原体，原文未明确写出]和/或[相关病原体，原文未明确写出]相关。在57.7% 的动物中发现了[相关病原体，原文未明确写出]，并且总是与另一种病原体一起被鉴定出来。对来自[相关病原体，原文未明确写出]阳性样本的96个样本中的[某种基因，原文未明确写出]基因进行测序，鉴定出了五个PilA组。大多数属于PilA组A。然而，鉴定出了三个新的PilA组，并命名为PilA组N、O和P。
结果表明，哈萨克斯坦东部畜牧场患有IBK的牛眼中[相关病原体，原文未明确写出]和[相关病原体，原文未明确写出]的感染率很高。还鉴定出了额外的新型[相关病原体，原文未明确写出]PilA组。
