Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran.
Department of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Microsc Res Tech. 2020 Dec;83(12):1552-1557. doi: 10.1002/jemt.23549. Epub 2020 Jul 31.
The application of nanoparticles in various industries has grown significantly in recent years. The aims of this study were evaluation effects of silver nanoparticles (AgNPs) on survival rate and tissues (Gills) of Guppy (Poecilia reticulate) as a model of the freshwater organism. For this purpose, 90 mature fish were exposed to a different level of AgNPs for 96 hr. Data analyzed showed there was a significant correlation between fish mortality rate and AgNPs concentrations. Histological assays showed some typical tissue damages such as hyperplasia, hypertrophy, hyperemia, hemorrhage, and necrosis. Moreover, fish exposed to a lethal concentration of AgNPs showed some clinical signs, such as increasing operculum movement, swimming near the water surface, anxiety, and death with open mouth. The results of the present study showed that AgNPs can have toxicity effects on Guppy, also to sub-lethal concentrations, leading to several tissue damages and reduced survival rate of fish.
近年来，纳米颗粒在各个行业中的应用有了显著的增长。本研究的目的是评估银纳米颗粒（AgNPs）对作为淡水生物模型的孔雀鱼（Poecilia reticulate）存活率和组织（鳃）的影响。为此，将 90 条成熟的鱼暴露于不同水平的 AgNPs 中 96 小时。数据分析显示，鱼死亡率与 AgNPs 浓度之间存在显著相关性。组织学分析显示出一些典型的组织损伤，如增生、肥大、充血、出血和坏死。此外，暴露于致死浓度 AgNPs 的鱼表现出一些临床症状，如增加鳃盖运动、在水面附近游动、焦虑和张嘴死亡。本研究结果表明，AgNPs 对孔雀鱼具有毒性作用，即使是亚致死浓度也会导致多种组织损伤和鱼类存活率降低。
