血液学研究中的单细胞谱系追踪方法：技术考量。
Single-cell lineage tracing approaches in hematology research: technical considerations.
机构信息
Haematopoietic Stem Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK; MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Immunology, The Walter & Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Faculty of Medicine, Dentistry & Health Sciences, University of Melbourne, Parkville, Victoria, Australia.
出版信息
Exp Hematol. 2020 Sep;89:26-36. doi: 10.1016/j.exphem.2020.07.007. Epub 2020 Jul 28.
The coordinated differentiation of hematopoietic stem and progenitor cells (HSPCs) into the various mature blood cell types is responsible for sustaining blood and immune system homeostasis. The cell fate decisions underlying this important biological process are made at the level of single cells. Methods to trace the fate of single cells are therefore essential for understanding hematopoietic system activity in health and disease and have had a major impact on how we understand and represent hematopoiesis. Here, we discuss the basic methodologies and technical considerations for three important clonal assays: single-cell transplantation, lentiviral barcoding, and Sleeping Beauty barcoding. This perspective is a synthesis of presentations and discussions from the 2019 International Society for Experimental Hematology (ISEH) Annual Meeting New Investigator Technology Session and the 2019 ISEH Winter Webinar.
造血干细胞和祖细胞（HSPCs）的协调分化为各种成熟的血细胞类型，负责维持血液和免疫系统的稳态。这一重要生物学过程中细胞命运的决定是在单细胞水平上做出的。因此，追踪单细胞命运的方法对于理解健康和疾病状态下造血系统的活动至关重要，并对我们理解和描述造血产生了重大影响。在这里，我们讨论了三种重要克隆分析方法的基本方法和技术考虑因素：单细胞移植、慢病毒条形码和睡眠美人条形码。本观点综合了 2019 年国际实验血液学学会（ISEH）年会新研究员技术会议和 2019 年 ISEH 冬季网络研讨会的演讲和讨论。
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