College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Key Laboratory for Processing and Transformation of Agricultural Products, Hubei, Wuhan Polytechnic University, Wuhan 430023, China.
Int J Biol Macromol. 2020 Dec 1;164:1413-1421. doi: 10.1016/j.ijbiomac.2020.07.217. Epub 2020 Jul 28.
Deoxynivalenol (DON) is the most common trichothecene distributed in food and feed. So far, much work has focused on investigating the cytotoxicity of DON, while there is few researches aimed at intervening in the toxic impacts on humans and livestock posed by DON. The objective of this study is to investigate the underlying mechanism of biomacromolecules mannan/β-glucans from yeast cell wall (BYCW) for their potency to impede the cytotoxicity and apoptosis caused by DON with porcine jejunum epithelial cell lines (IPEC-J2) used as a cell injury model. We analyzed the cell morphology, cell activity, oxidative stress, fluorescence intensity and expressions of proteins relevant to autophagy, apoptosis and PI3K-AKT-mTOR signaling pathway by using inverted microscopy, MTS, reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) assay, Annexin V-FITC / propidium iodide (PI) double staining and Western blot assay. The consequent data demonstrated that in the presence of BYCW, the cell morphology and activity were relatively ameliorated and that the oxidation damage was attenuated with DON-induced autophagy concomitantly decreased, which, furthermore, was found involved in the positive regulation on PI3K-AKT-mTOR signaling pathway by BYCW. In a word, BYCW possess an ability to repress the cytotoxicity and apoptosis induced by DON through the inhibition of autophagy via activating PI3K-AKT-mTOR signaling pathway.
脱氧雪腐镰刀菌烯醇（DON）是分布在食品和饲料中最常见的一种单端孢霉烯族化合物。到目前为止，大量的工作都集中在研究 DON 的细胞毒性，而很少有研究旨在干预 DON 对人类和牲畜的毒性影响。本研究旨在探讨酵母细胞壁甘露聚糖/β-葡聚糖（BYCW）作为一种细胞损伤模型，对 DON 诱导的细胞毒性和细胞凋亡的潜在作用机制。我们通过倒置显微镜、MTS、活性氧（ROS）、谷胱甘肽（GSH）和丙二醛（MDA）测定、Annexin V-FITC/碘化丙啶（PI）双重染色和 Western blot 分析，研究了细胞形态、细胞活性、氧化应激、荧光强度以及与自噬、细胞凋亡和 PI3K-AKT-mTOR 信号通路相关的蛋白质表达。结果表明，在 BYCW 的存在下，细胞形态和活性得到了相对改善，DON 诱导的自噬减少，氧化损伤减轻，进一步证实 BYCW 通过激活 PI3K-AKT-mTOR 信号通路对自噬产生正向调节作用。总之，BYCW 通过抑制自噬来抑制 DON 诱导的细胞毒性和细胞凋亡，激活 PI3K-AKT-mTOR 信号通路。
