在 Sulforhodamine B 结合 RNA 适体中的配体特异性和亲和力。
Ligand specificity and affinity in the sulforhodamine B binding RNA aptamer.
机构信息
Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.
Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada; Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.
出版信息
Biochem Biophys Res Commun. 2020 Aug 27;529(3):666-671. doi: 10.1016/j.bbrc.2020.06.056. Epub 2020 Jul 18.
Binding affinity and selectivity are critical properties of aptamers that must be optimized for any application. The sulforhodamine B binding RNA aptamer (SRB-2) is a somewhat promiscuous aptamer that can bind ligands that vary markedly in shape, size and charge. Here we categorize potential ligands based on their binding mode and structural characteristics required for high affinity and selectivity. Several known and potential ligands of SRB-2 were screened for binding affinity using LSPR, ITC and NMR spectroscopy. The study shows that rhodamine B has the ideal structural and electrostatic properties for selective and high-affinity binding of the SRB-2 aptamer.
结合亲和力和选择性是适体的关键性质，对于任何应用都必须进行优化。吖啶黄结合 RNA 适体（SRB-2）是一种有些混杂的适体，可以结合在形状、大小和电荷上差异显著的配体。在这里，我们根据结合模式和高亲和力和选择性所需的结构特征对潜在配体进行分类。使用 LSPR、ITC 和 NMR 光谱法筛选了几种已知和潜在的 SRB-2 配体的结合亲和力。该研究表明，吖啶黄具有理想的结构和静电特性，可选择性和高亲和力结合 SRB-2 适体。
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