Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga, Japan.
Research Organization of Science and Technology, Ritsumeikan University, Kusatsu, Shiga, Japan.
Biochem Biophys Res Commun. 2020 Aug 27;529(3):733-739. doi: 10.1016/j.bbrc.2020.05.205. Epub 2020 Jul 19.
The vitamin D pathway is related to the mass and function of skeletal muscles. Several studies have demonstrated the role of vitamin D receptor (VDR) and CYP27B1 in skeletal muscles, suggesting that these proteins may regulate skeletal muscles and their function. However, it remains unclear whether the expression of VDR and CYP27B1 is modified in skeletal muscle atrophy. We investigated whether denervation-induced muscle atrophy is associated with altered expression of VDR and CYP27B1 in murine skeletal muscles. Skeletal muscles were excised from C57BL/6J mice, 3 and 7 days after the mice underwent denervation surgery. Denervation induced muscle atrophy and enhanced the expression of MuRF1 and Atrogin-1 in the gastrocnemius and soleus. The protein expression of VDR was increased in the denervated gastrocnemius; in contrast, denervation decreased the protein expression of CYP27B1 in the gastrocnemius and soleus. These results suggest that denervation-induced muscle atrophy is associated with changes in the expression of vitamin D-related proteins in murine skeletal muscles.
维生素 D 通路与骨骼肌的质量和功能有关。多项研究表明维生素 D 受体 (VDR) 和 CYP27B1 在骨骼肌中的作用，提示这些蛋白可能调节骨骼肌及其功能。然而，VDR 和 CYP27B1 的表达是否在骨骼肌萎缩中发生改变仍不清楚。我们研究了去神经诱导的肌肉萎缩是否与小鼠骨骼肌中 VDR 和 CYP27B1 表达的改变有关。在小鼠接受去神经手术后 3 天和 7 天，从 C57BL/6J 小鼠中切除骨骼肌。去神经导致肌肉萎缩，并增强了腓肠肌和比目鱼肌中 MuRF1 和 Atrogin-1 的表达。VDR 的蛋白表达在去神经的腓肠肌中增加；相反，去神经降低了腓肠肌和比目鱼肌中 CYP27B1 的蛋白表达。这些结果表明，去神经诱导的肌肉萎缩与小鼠骨骼肌中维生素 D 相关蛋白表达的变化有关。
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