Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, RS, 97500-970, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, RS, 97500-970, Brazil.
Food Chem Toxicol. 2020 Oct;144:111625. doi: 10.1016/j.fct.2020.111625. Epub 2020 Jul 29.
This study was designed to examine fetal and maternal toxicity of curcumin (CURC) loaded lipid-core nanocapsules (LNC) prepared with poly(ϵ-caprolactone) as a polymer, administered during the organogenesis period. Free CURC and CURC loaded-LNC (C-LNC) (2 mg/kg), blank LNC (B-LNC) and saline (CONTROL) were administered per oral route from the 7° to 13° gestational day (GD). Dams were evaluated daily for body weight gain, clinical signs, water and food intake. On 20° GD, dams were euthanized, organs were weighed and blood was collected for biochemical determinations. Fetal biometrics and external morphological anomalies were assessed. Also, were performed histopathological analysis of placenta and measurement of cytokines levels in placental and fetal liver tissues. All groups did not cause changes in dams during the pregnancy. Furthermore, treatments did not cause external morphological changes and delayed fetal development. Still, for histopathological analysis of placental tissue, treatments did not cause alterations in evaluated parameters. For cytokines levels, CURC and C-LNC caused a decrease in placental levels of TNF-α. Therefore, we have demonstrated that C-LNC did not cause toxicological effects (mother and fetus), in the same manner as pattern bioactive compound, proving to be a promising nutraceutical delivery system for maternal supplementation with CURC.
本研究旨在考察聚己内酯作为聚合物制备的载姜黄素（CURC）脂质核纳米胶囊（LNC）在器官发生期给药时的胎儿和母体毒性。游离 CURC 和载 CURC 的 LNC（C-LNC）（2mg/kg）、空白 LNC（B-LNC）和生理盐水（CONTROL）经口给予 7°至 13°孕日（GD）。每天评估母体体重增加、临床症状、水和食物摄入情况。在 20°GD 时，处死母体，称重器官并采集血液进行生化测定。评估胎儿生物测量和外部形态异常。还对胎盘进行组织病理学分析，并测量胎盘和胎肝组织中细胞因子的水平。所有组在怀孕期间均未引起母体变化。此外，这些处理未引起外部形态变化和胎儿发育延迟。尽管如此，对于胎盘组织的组织病理学分析，处理并未引起评估参数的改变。对于细胞因子水平，CURC 和 C-LNC 导致胎盘 TNF-α水平降低。因此，我们已经证明 C-LNC 没有引起毒理学效应（母体和胎儿），与模式生物活性化合物一样，证明它是一种有前途的母体用 CURC 补充的营养输送系统。
