研制一种侧向流测试条，用于同时检测转基因作物中的 BT-Cry1Ab、BT-Cry1Ac 和 CP4 EPSPS 蛋白。
Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops.
机构信息
The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
出版信息
Food Chem. 2021 Jan 15;335:127627. doi: 10.1016/j.foodchem.2020.127627. Epub 2020 Jul 25.
A colloidal gold immunochromatographic strip (ICS) for simultaneous detection of multiple transgenic proteins, including CP4 EPSPS, BT-Cry1Ab and BT-Cry1Ac, was developed in this study. The sensitivity of the strip to the target protein was 5 ng/mL for CP4 EPSPS, 100 ng/mL for BT-Cry1Ab and Cry1Ac, respectively. Parallel analysis for maize, soybean, sugar beet and cotton showed the strip could detect 1% of transgenic content in crops containing BT-Cry1Ab and Cry1Ac, and, at least, 0.1% of content in crops containing CP4 EPSPS. The detection results for seed samples indicated the multicomponent analysis ICS had good accuracy. The analysis could be completed within 10 min and had the advantages of being high-throughput, easy to operate and visual detection. This is the first report of semi-quantitative ICS for detecting three transgenic proteins simultaneously. The developed approach may provide insights into the development of ICS for analyzing simultaneously multiple components in genetically modified crops.
本研究开发了一种胶体金免疫层析条（ICS），可同时检测多种转基因蛋白，包括 CP4 EPSPS、BT-Cry1Ab 和 BT-Cry1Ac。该条带对目标蛋白的灵敏度分别为 CP4 EPSPS 为 5ng/mL，BT-Cry1Ab 和 Cry1Ac 为 100ng/mL。对玉米、大豆、甜菜和棉花的平行分析表明，该条带可检测到含有 BT-Cry1Ab 和 Cry1Ac 的作物中转基因含量的 1%，以及至少含有 CP4 EPSPS 的作物中转基因含量的 0.1%。种子样品的检测结果表明，该多组分分析 ICS 具有良好的准确性。分析可在 10 分钟内完成，具有高通量、易于操作和可视化检测的优点。这是首次报道半定量 ICS 同时检测三种转基因蛋白。所开发的方法可能为开发用于分析转基因作物中多种成分的 ICS 提供思路。
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