Department of Microbiology, Savitribai Phule Pune University, Pune, 411007, Maharashtra, India.
CSIR-National Chemical Laboratory, Organic Chemistry Division, Pune, 411008, Maharashtra, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
J Trace Elem Med Biol. 2020 Dec;62:126630. doi: 10.1016/j.jtemb.2020.126630. Epub 2020 Jul 24.
Biogenic nanoparticles are gaining attention due to their low toxicity and numerous biomedical applications. Present study aimed to compare the potential anticancer activity of two biogenic silver nanoparticles (bAgNPs and pAgNPs) against human cervical cancer cell lines (HeLa).
bAgNPs were synthesized using Acinetobacter sp. whereas pAgNPs were synthesized using aqueous root extract of Curcuma aromatica. Effect of these nanoparticles on HeLa cells viability was studied using MTT assay and colony formation assay. Anticancer potential was determined using fluorescence microscopy and flow cytometry studies. Bio-compatibility studies were performed against peripheral blood mononuclear cells (PBMCs).
Both the nanoparticles showed 50 % viability of peripheral blood mononuclear cells (PBMCs) when used at high concentration (200 μg/mL). IC for bAgNPs and pAgNPs against HeLa cells were 17.4 and 14 μg/mL respectively. Colony formation ability of Hela cells was reduced on treatment with both nanoparticles. Acridine orange and ethidium bromide staining demonstrated that bAgNPs were cytostatic whereas pAgNPs were apoptotic. JC-1 dye staining revealed that the mitochondrial membrane potential was affected on treatment with pAgNPs while it remained unchanged on bAgNPs treatment. Flow cytometry confirmed cell cycle arrest in HeLa cells on treatment with nanoparticles further leading to apoptosis in case of pAgNPs. About 77 and 58 % HeLa cells were found in subG1 phase on treatment with bAgNPs and pAgNPs respectively. bAgNPs showed cytostatic effect on HeLa cells arresting the cell growth in subG1 phase, whereas, pAgNPs triggered death of HeLa cells through mitochondrial membrane potential impairment and apoptosis.
Overall, bAgNPs and pAgNPs could be safe and showed potential to be used as anticancer nano-antibiotics against human cervical cancer cells.
由于生物成因纳米颗粒的低毒性和众多生物医学应用，它们受到了广泛关注。本研究旨在比较两种生物成因银纳米颗粒（bAgNPs 和 pAgNPs）对人宫颈癌细胞系（HeLa）的潜在抗癌活性。
bAgNPs 是使用不动杆菌属合成的，而 pAgNPs 是使用姜黄的水根提取物合成的。使用 MTT 测定法和集落形成测定法研究这些纳米颗粒对 HeLa 细胞活力的影响。通过荧光显微镜和流式细胞术研究确定抗癌潜力。对外周血单核细胞（PBMCs）进行生物相容性研究。
当使用高浓度（200μg/mL）时，两种纳米颗粒对外周血单核细胞（PBMCs）的存活率均为 50%。bAgNPs 和 pAgNPs 对 HeLa 细胞的 IC 分别为 17.4 和 14μg/mL。用两种纳米颗粒处理后，Hela 细胞的集落形成能力降低。吖啶橙和溴化乙锭染色表明 bAgNPs 是细胞停滞剂，而 pAgNPs 是凋亡剂。JC-1 染料染色表明，pAgNPs 处理后线粒体膜电位受到影响，而 bAgNPs 处理后保持不变。流式细胞术证实纳米颗粒处理后 HeLa 细胞周期停滞，进一步导致 pAgNPs 凋亡。用 bAgNPs 和 pAgNPs 处理后，约有 77%和 58%的 HeLa 细胞分别处于 subG1 期。bAgNPs 对 HeLa 细胞具有细胞停滞作用，使细胞生长停滞在 subG1 期，而 pAgNPs 通过线粒体膜电位损伤和凋亡引发 HeLa 细胞死亡。
总的来说，bAgNPs 和 pAgNPs 可能是安全的，并显示出作为针对人宫颈癌细胞的抗癌纳米抗生素的潜力。
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