Biomaterials - BIOMAT, Department of Oral Health Sciences, KU Leuven, Leuven, Belgium.
Prometheus - Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium.
Calcif Tissue Int. 2020 Oct;107(4):371-380. doi: 10.1007/s00223-020-00732-0. Epub 2020 Aug 1.
Obesity is the main cause of type 2 diabetes mellitus (T2DM). Roux-en-Y gastric bypass (RYGB) surgery is an effective treatment for this obesity-related health problem. However, the adverse effects of T2DM on bone tissue persist or even aggravate after this surgical procedure. As studies on the mandibular condyle bone are scarce, the aim of the present study was to assess its compositional characteristics in T2DM and RYGB conditions. Thirty-two male C57BL/6 mice at 8 weeks of age were randomly assigned to receive either a high-fat or low-fat diet. After 14 weeks of high-fat diet intake, seven obese mice were subjected to RYGB surgery. All animals were euthanized at the age of 30 weeks. Mandibular bones were removed and the trabecular condyle region was assessed using Raman spectroscopy. A decreased mineralization was observed for both T2DM and RYGB condyle bones when compared to controls, with elevated carbonate substitutions for the RYGB group. No compositional differences in crystallinity and presence of advanced glycation end products were found between the groups, with the exception of an increased presence of N-carboxymethyl-lysine in RYGB bone compared to their T2DM counterpart. Site-specific measurements revealed a non-uniform bone composition, with increasing mineralization and carbonate substitutions towards the centre of the mandibular condyle. T2DM and RYGB surgery affect the mandibular condyle bone quality, as investigated at compositional level. Assessment of bone structural properties and remodelling should be carried out to further explore the effects of T2DM and RYGB surgery on this skeleton area.
肥胖是 2 型糖尿病（T2DM）的主要原因。Roux-en-Y 胃旁路（RYGB）手术是治疗这种肥胖相关健康问题的有效方法。然而，T2DM 对骨骼组织的不良影响在手术后仍然存在，甚至加重。由于对下颌髁骨的研究较少，本研究旨在评估 T2DM 和 RYGB 状态下其组成特征。32 只 8 周龄雄性 C57BL/6 小鼠被随机分为高脂或低脂饮食组。高脂饮食摄入 14 周后，7 只肥胖小鼠接受 RYGB 手术。所有动物在 30 周龄时被安乐死。取出下颌骨，使用拉曼光谱评估髁突小梁区。与对照组相比，T2DM 和 RYGB 髁突骨的矿化程度降低，RYGB 组的碳酸盐取代物升高。各组之间在结晶度和晚期糖基化终产物的存在方面没有发现组成差异，但 RYGB 骨中 N-羧甲基赖氨酸的存在增加，与 T2DM 骨相比。特定部位的测量显示出不均匀的骨组成，随着向髁突中心的推进，矿化程度和碳酸盐取代物增加。T2DM 和 RYGB 手术影响下颌髁骨质量，在组成水平上进行了研究。应该进行骨结构特性和重塑的评估，以进一步探讨 T2DM 和 RYGB 手术对该骨骼区域的影响。
