Department of Environmental and Molecular Health Sciences, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University.
Institute for Bee Products and Health Science, Yamada Bee Company, Inc.
Biol Pharm Bull. 2020;43(8):1202-1209. doi: 10.1248/bpb.b20-00101.
The effective antigen (Ag) uptake by microfold cells (M-cells) is important for the induction of an efficient mucosal immune responses. Here, we show that 10-hydroxydecanoic acid (10-HDAA) from royal jelly (RJ) potentially supports M-cell differentiation and induces effective antigen-specific mucosal immune responses in cynomolgus macaques. 10-HDAA increases the expression level of receptor activator of nuclear factor-kappaB (NF-κB) (RANK) in Caco-2 cells, which suggests that 10-HDAA potentially prompts the differentiation of Caco-2 cells into M-cells and increased transcytosis efficiency. This idea is supported by the following observations. Intranasal administration of 10-HDAA increased the number of M-cells in the epithelium overlying nasopharynx-associated lymphoid tissue (NALT) in macaques. Oral administration of 10-HDAA increased the number of M-cells in the follicle-associated epithelium (FAE) covering Peyer's patches (PPs) and significantly increased the antigen-specific immunoglobulin A (IgA) level in macaques. These findings suggest that the exogenous honeybee-derived medium-chain fatty acid 10-HDAA may effectively enhance antigen-specific immune responses.
有效抗原（Ag）被黏膜褶内细胞（M 细胞）摄取对于诱导有效的黏膜免疫反应非常重要。在这里，我们表明，蜂王浆（RJ）中的 10-羟基癸酸（10-HDAA）可能支持 M 细胞分化，并在食蟹猴中诱导有效的抗原特异性黏膜免疫反应。10-HDAA 增加了核因子-κB（NF-κB）受体激活剂（RANK）在 Caco-2 细胞中的表达水平，这表明 10-HDAA 可能促使 Caco-2 细胞分化为 M 细胞，并增加了转胞吞作用效率。这一观点得到了以下观察结果的支持。鼻内给予 10-HDAA 增加了猴鼻咽相关淋巴组织（NALT）上皮中 M 细胞的数量。口服给予 10-HDAA 增加了覆盖派伊尔氏斑（PP）的滤泡相关上皮（FAE）中 M 细胞的数量，并显著增加了猴体内抗原特异性免疫球蛋白 A（IgA）的水平。这些发现表明，外源性蜜蜂源性中链脂肪酸 10-HDAA 可能有效地增强抗原特异性免疫反应。
