Wang Dan-Wen, Su Fei, Zhang Tao, Yang Tie-Cheng, Wang Hua-Qiao, Yang Li-Jie, Zhou Fen-Fang, Feng Mao-Hui
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, People's Republic of China.
Center for Clinical Medicine of Peritoneal Cancer of Wuhan, Wuhan 430071, Hubei Province, People's Republic of China.
J Cancer. 2020 Jun 23;11(17):5042-5055. doi: 10.7150/jca.45553. eCollection 2020.
Ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) is an important mitochondrial complex III subunit. This study investigated the role of UQCRC2 in gastric cancer (GC) and its upstream regulatory microRNAs (miRNAs). UQCRC2 expression levels were lower in GC tissues than non-carcinoma tissues. Furthermore, UQCRC2 levels were negatively correlated with lymph node metastasis, relapse, and tumor grade. Bioinformatics analysis predicted UQCRC2 as the target gene for and this was verified in luciferase reporter assays. levels were inversely correlated with UQCRC2 levels in GC. UQCRC2 overexpression suppressed GC cell migration and invasion and whereas up-regulating reversed these effects. Western blotting analysis showed that targeted UQCRC2 and positively regulated the epithelial-mesenchymal transition (EMT) signaling pathway in GC cells. Therefore, the /UQCRC2 axis may regulate EMT signaling pathways to affect tumor proliferation and metastasis and is, thus, a potential target for GC treatment.
泛醇 - 细胞色素c还原酶核心蛋白2（UQCRC2）是线粒体复合物III的一个重要亚基。本研究调查了UQCRC2在胃癌（GC）中的作用及其上游调控微小RNA（miRNA）。UQCRC2在GC组织中的表达水平低于非癌组织。此外，UQCRC2水平与淋巴结转移、复发及肿瘤分级呈负相关。生物信息学分析预测UQCRC2为[具体miRNA名称缺失]的靶基因，荧光素酶报告基因检测验证了这一点。[具体miRNA名称缺失]水平在GC中与UQCRC2水平呈负相关。UQCRC2过表达抑制GC细胞迁移和侵袭，而上调[具体miRNA名称缺失]则逆转了这些作用。蛋白质免疫印迹分析表明，[具体miRNA名称缺失]靶向UQCRC2并正向调控GC细胞中的上皮 - 间质转化（EMT）信号通路。因此，[具体miRNA名称缺失]/UQCRC2轴可能调控EMT信号通路以影响肿瘤增殖和转移，故而可能是GC治疗的一个潜在靶点。
