Lang M J, Chung M T, Tu Y C
Zhonghua Ya Yi Xue Hui Za Zhi. 1988 Sep;7(3):113-27.
The expression of cytokeratin (40-52 kD), carcinoembryonic antigen (CEA), vimentin, neuron-specific enolase (NSE), S-100 protein, glial fibrillary acidic protein (GFAP) were investigated in 20 cases of pleomorphic adenoma of the salivary glands and 10 cases of normal salivary glands, in order to analyze and correlate the antigens' expressions with the probably histogenetic mechanisms of the various histopathological differentiations in pleomorphic adenoma of the salivary glands and their probably original cells in normal salivary glands. Immunohistochemistry has provided some evidence for the relationship of the tumor cells to normal salivary glands: In the normal glands, the acinic cells exhibited cytokeratin, CEA and focal, predominantly nuclear S-100 protein staining. In both normal glands and pleomorphic adenomas, the duct-lining cells were immunoreactive for cytokeratin, CEA and had both cytoplasmic and nuclear S-100 positivity; The myoepithelial cells of the normal glands as well as the periduct cells, epithelial nests/cords, squamous metaplasia and the stellate/spindle/cartilaginous cells in the myxomatous-chondroid areas of the pleomorphic adenoma contain immunoreactive vimentin, NSE, S-100 proteins and GFAP, and lesser amounts cytokeratin (40-52 kD)/CEA. The varicosities of the terminal axon may lie directly on the basal membrane, or penetrate the basal membrane and lie in direct contact with the effector cells (duct-acinar-myoepithelial cells) of the salivary glands. The peripheral neurons and axons of the autonomic nervous system were identified by vimentin, NSE, S-100 proteins and GFAP. The combination of epithelial cytokeratin and nervous system-related vimentin, NSE, S-100 and GFAP immunostaining in myoepithelium of the normal glands and in all component elements (particularly the periduct cells) of pleomorphic adenoma reflects pleomorphic adenoma of the salivary glands is an epithelial tumor, the probably original cells or the probably histogenetic mechanisms of the various histopathological differentiations is correlated not only with "duct-acinar-myoepithelial cells" but also with the neuroectoderm in the normal salivary glands."
研究了20例涎腺多形性腺瘤和10例正常涎腺中细胞角蛋白（40 - 52 kD）、癌胚抗原（CEA）、波形蛋白、神经元特异性烯醇化酶（NSE）、S - 100蛋白、胶质纤维酸性蛋白（GFAP）的表达情况，以分析这些抗原的表达与涎腺多形性腺瘤各种组织病理学分化的可能组织发生机制及其在正常涎腺中可能的原始细胞之间的关系。免疫组织化学为肿瘤细胞与正常涎腺的关系提供了一些证据：在正常腺体中，腺泡细胞表现出细胞角蛋白、CEA以及局灶性、主要为核内的S - 100蛋白染色。在正常腺体和多形性腺瘤中，导管内衬细胞对细胞角蛋白、CEA呈免疫反应性，且细胞质和细胞核S - 100均呈阳性；正常腺体的肌上皮细胞以及多形性腺瘤黏液样软骨样区域的导管周围细胞、上皮巢/索、鳞状化生及星状/梭形/软骨样细胞含有免疫反应性波形蛋白、NSE、S - 100蛋白和GFAP，以及少量细胞角蛋白（40 - 52 kD）/CEA。终末轴突的曲张可能直接位于基底膜上，或穿透基底膜并与涎腺的效应细胞（导管 - 腺泡 - 肌上皮细胞）直接接触。自主神经系统的外周神经元和轴突通过波形蛋白、NSE、S - 100蛋白和GFAP得以识别。正常腺体肌上皮以及多形性腺瘤所有组成成分（尤其是导管周围细胞）中上皮细胞角蛋白与神经系统相关的波形蛋白、NSE、S - 100和GFAP免疫染色的结合反映出涎腺多形性腺瘤是一种上皮性肿瘤，各种组织病理学分化的可能原始细胞或可能组织发生机制不仅与 “导管 - 腺泡 - 肌上皮细胞” 相关，还与正常涎腺中的神经外胚层相关。
