Zhao Qinli, Zheng Xiwang, Guo Huina, Xue Xuting, Zhang Yuliang, Niu Min, Cui Jiajia, Liu Hongliang, Luo Hongjie, Yang Dongli, Shi Yong, Huangfu Hui, Wen Shuxin, Wu Yongyan, Gao Wei, Wang Binquan
Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.
J Cancer. 2020 Jul 9;11(18):5329-5344. doi: 10.7150/jca.45394. eCollection 2020.
At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate serum exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a high-yield and is a highly efficient exosome isolation method, was selected to isolate serum exosomes in the current study. In LSCC samples, exosome concentrations were higher than in healthy control (HC) samples. RNA-seq analysis identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is significantly upregulated in LSCC serum exosomes, with this same trend seen in LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 overexpression promoted proliferation and invasion, while miR-941 knockdown inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may serve as a promising oncogenic biomarker for diagnosing LSCC, and has the potential as a therapeutic target.
目前，尚无基于血液的生物标志物用于喉鳞状细胞癌（LSCC）的临床实践。越来越多的证据表明，循环外泌体微小RNA（miRNA）可能作为各种癌症的潜在诊断生物标志物。本研究旨在鉴定和评估用于LSCC诊断的血清外泌体miRNA。在本研究中，选择了能提供高产率且高效的外泌体分离方法的ExoQuick溶液（EQ）来分离血清外泌体。在LSCC样本中，外泌体浓度高于健康对照（HC）样本。RNA测序分析共鉴定出1608种miRNA，与HC样本相比，LSCC样本中有34种上调，41种下调。此外，qRT-PCR显示miR-941在LSCC血清外泌体中显著上调，在LSCC组织和细胞中也呈现相同趋势。此外，在细胞系中检测miR-941时，miR-941过表达促进增殖和侵袭，而miR-941敲低则抑制细胞增殖和侵袭。ROC曲线分析表明，miR-941区分LSCC患者和HC的曲线下面积（AUC）为0.797（95%CI = 0.676 - 0.918）。总之，血清外泌体miR-941可能是诊断LSCC的一种有前景的致癌生物标志物，并有作为治疗靶点的潜力。
