Tian Meiju, Wang Chunyan, Ma Qiujuan, Bai Yu, Sun Jingguo, Ding Chunfeng
School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, PR China.
Zhengzhou Key Laboratory of Chinese Medicine Quality Control and Evaluation, Zhengzhou 450046, PR China.
ACS Omega. 2020 Jul 14;5(29):18176-18184. doi: 10.1021/acsomega.0c01790. eCollection 2020 Jul 28.
Hg has a significant hazardous impact on the environment and ecosystem. There is a great demand for new methods with high selectivity and sensitivity to determine mercury in life systems and environments. In this paper, a novel turn-on Hg fluorescent probe has been reported with a naphthalimide group. The Hg fluorescent probe was designed by the inspiration of the well-known specific Hg-triggered thioacetal deprotection reaction. A 1,2-dithioalkyl group was chosen as the specific recognition site of Hg. The probe showed weak fluorescence without Hg, and the color of the solution was light yellow. In the presence of Hg, the probe reacted specifically with the mercury ion to produce an aldehyde and emitted strong fluorescence, and the color of the solution also turned light green, thus realizing the monitoring of the mercury ion. The Hg fluorescent probe showed outstanding sensitivity and selectivity toward Hg. Furthermore, the Hg fluorescent probe could work in a wide pH range. The linear relationship between the fluorescence intensity at 510 nm and the concentration of Hg was obtained in a range of Hg concentration from 2.5 × 10 to 1.0 × 10 M. The detection limit was found to be 4.0 × 10 M for Hg. Furthermore, with little cell toxicity, the probe was successfully applied to the confocal image of Hg in PC-12 cells.
汞对环境和生态系统具有重大危害影响。对于在生命系统和环境中测定汞的具有高选择性和灵敏度的新方法有很大需求。本文报道了一种带有萘酰亚胺基团的新型开启式汞荧光探针。该汞荧光探针是受著名的特定汞引发的硫代缩醛脱保护反应启发而设计的。选择1,2 - 二硫代烷基作为汞的特异性识别位点。该探针在没有汞的情况下显示出微弱荧光，溶液颜色为浅黄色。在有汞存在时，探针与汞离子特异性反应生成醛并发出强荧光，溶液颜色也变为浅绿色，从而实现了对汞离子的监测。该汞荧光探针对汞表现出出色的灵敏度和选择性。此外，该汞荧光探针可在较宽的pH范围内工作。在汞浓度从2.5×10到1.0×10 M的范围内，获得了510 nm处荧光强度与汞浓度之间的线性关系。发现汞的检测限为4.0×10 M。此外，该探针细胞毒性小，已成功应用于PC - 12细胞中汞的共聚焦成像。
