Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, South-Central University for Nationalities, Wuhan, 430074, China.
School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China.
Mikrochim Acta. 2020 Aug 2;187(8):480. doi: 10.1007/s00604-020-04411-7.
MoS nanosheets were prepared by exfoliating MoS bulk crystals with ultrasonication in N-methylpyrrolidone and were integrated with gold nanostars (AuNS) to fabricate an AuNS/MoS nanocomposite. All nanomaterials were characterized by transmission electron microscope, scanning electron microscope, ultraviolet-visible spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. AuNS/MoS nanocomposites were coated onto a glassy carbon electrode (GCE) surface to construct a nanointerface for immobilizing neuron-specific enolase antibody (anti-NSE) thus forming a photoelectrochemical immunoassay system. AuNS can significantly promote the photoelectric conversion of MoS nanosheets improving the performance for a photoelectrochemical assay. Being illuminated with white light LED and controlling the potential at 0.05 V (vs. SCE), the photocurrent generated from anti-NSE(BSA)/AuNS/MoS/GCE using 0.15 mol L ascorbic acid as electron donor can be recorded with amperometry and used as an output signal for NSE quantitative assay. Under optimized experimental conditions, the photocurrent variation for the affinity-binding NSE is proportional to the logarithm of NSE concentration in the range 5.0 pg mLto 1.5 ng mL with a detection limit of 3.5 pg mL (S/N = 3). The practicability of the PEC immunoassay system was evaluated by determining NSE in clinical serum samples. The recoveries ranged from 93.0 to 103% for the determination of NSE in serum samples with a standard addition method. The PEC immunoassay system possesses good accuracy for determining NSE in real samples. Graphical abstract.
MoS 纳米片通过在 N-甲基吡咯烷酮中超声剥离 MoS 块状晶体制备，并与金纳米星（AuNS）集成，以制备 AuNS/MoS 纳米复合材料。所有纳米材料均通过透射电子显微镜、扫描电子显微镜、紫外-可见光谱、X 射线衍射和 X 射线光电子能谱进行了表征。AuNS/MoS 纳米复合材料涂覆在玻碳电极（GCE）表面上，构建了用于固定神经元特异性烯醇化酶抗体（抗-NSE）的纳米界面，从而形成了光电化学免疫分析系统。AuNS 可以显著促进 MoS 纳米片的光电转换，提高光电化学分析的性能。在 0.05 V（相对于 SCE）的电位下用白光 LED 照射，并记录使用 0.15 mol L 抗坏血酸作为电子供体的抗-NSE（BSA）/AuNS/MoS/GCE 产生的光电流，可作为 NSE 定量分析的输出信号。在优化的实验条件下，亲和结合 NSE 的光电流变化与 NSE 浓度在 5.0 pg mL 至 1.5 ng mL 范围内的对数成正比，检测限为 3.5 pg mL（S/N = 3）。通过用标准添加法测定血清样品中的 NSE 来评估 PEC 免疫分析系统的实用性。用于测定血清中 NSE 的回收率范围为 93.0%至 103%。PEC 免疫分析系统用于测定实际样品中 NSE 的准确性良好。
