Yu Xiangyang, Wang Yanying, Chen Xuemin, Wu Kangbing, Chen Danchao, Ma Ming, Huang Zhenjia, Wu Wangze, Li Chunya
†Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
‡Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Anal Chem. 2015 Apr 21;87(8):4237-44. doi: 10.1021/ac504456w. Epub 2015 Mar 31.
ZnCdHgSe quantum dots (QDs) functionalized with N-acetyl-l-cysteine were synthesized and characterized. Through layer-by-layer assembling, the ZnCdHgSe QDs was integrated with a polymerized 1-decyl-3-[3-pyrrole-1-yl-propyl]imidazolium tetrafluoroborate (PDPIT) ionic liquid film modified indium tin oxide (ITO) electrode to fabricated a photoelectrochemical interface for the immobilization of rabbit antihuman neuron specific enolase (anti-NSE). After being treated with glutaraldehyde vapor and bovine serum albumin successively, an anti-NSE/ZnCdHgSe QDs/PDPIT/ITO sensing platform was established. Simplely using a white-light LED as an excitation source, the immunoassay of neuron specific enolase (NSE) was achieved through monitoring the photocurrent variation. The polymerized ionic liquid film was demonstrated to be an important element to enhance the photocurrent response of ZnCdHgSe QDs. The anti-NSE/ZnCdHgSe QDs/PDPIT/ITO based immunosensor presents excellent performances in neuron specific enolase determination. The photocurrent variation before and after being interacted with NSE exhibits a good linear relationship with the logarithm of its concentration (log cNSE) in the range from 1.0 pg mL(-1) to 100 ng mL(-1). The limit of detection of this immunosensor is able to reach 0.2 pg mL(-1) (S/N = 3). The determination of NSE in clinical human sera was also demonstrated using anti-NSE/ZnCdHgSe QDs/PDPIT/ITO electrode. The results were found comparable with those obtained by using enzyme-linked immunosorbent assay method.
合成并表征了用N-乙酰基-L-半胱氨酸功能化的ZnCdHgSe量子点（QDs）。通过层层组装，将ZnCdHgSe量子点与聚合的1-癸基-3-[3-吡咯-1-基丙基]咪唑四氟硼酸盐（PDPIT）离子液体膜修饰的氧化铟锡（ITO）电极集成，制备了用于固定兔抗人神经元特异性烯醇化酶（抗NSE）的光电化学界面。依次用戊二醛蒸气和牛血清白蛋白处理后，建立了抗NSE/ZnCdHgSe QDs/PDPIT/ITO传感平台。仅使用白光发光二极管作为激发源，通过监测光电流变化实现了神经元特异性烯醇化酶（NSE）的免疫测定。结果表明，聚合离子液体膜是增强ZnCdHgSe量子点光电流响应的重要元素。基于抗NSE/ZnCdHgSe QDs/PDPIT/ITO的免疫传感器在神经元特异性烯醇化酶测定中表现出优异的性能。与NSE相互作用前后的光电流变化与其浓度的对数（log cNSE）在1.0 pg mL(-1)至100 ng mL(-1)范围内呈现良好的线性关系。该免疫传感器的检测限能够达到0.2 pg mL(-1)（S/N = 3）。还使用抗NSE/ZnCdHgSe QDs/PDPIT/ITO电极对临床人血清中的NSE进行了测定。结果发现与使用酶联免疫吸附测定法获得的结果相当。
