印楝叶和果实的甲醇提取物对大鼠精子质量和睾丸组织学表现出抗生育活性。

Leaf and Fruit Methanolic Extracts of Azadirachta indica Exhibit Antifertility Activity on Rats' Sperm Quality and Testicular Histology.

机构信息

Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Mexico.

Department of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Centre of Bioengineering, Campus Queretaro, Queretaro, Mexico.

出版信息

Curr Pharm Biotechnol. 2021;22(3):400-407. doi: 10.2174/1389201021666200730145621.

DOI:10.2174/1389201021666200730145621

PMID:32744965

Abstract

BACKGROUND

The world's population is still growing, having an impact on the environment and the economic growth of developing countries; so that, there is a particular interest in the development of new fertility control methods, focused on male contraception.

OBJECTIVE

The objective of this study was to evaluate the effect of methanolic extracts of leaf and fruit of Azadirachta indica on sperm quality and testicular histology of Long Evans rats.

METHODS

Antifertility effects of a methanolic leaf and fruit extracts of A. indica on 24 male rats were investigated. The animals were randomly divided into two control groups and four treatment groups (n=4). Doses of the leaf and fruit extract were given at concentrations of 100 and 200 μg mL-1.

RESULTS

A significant decrease in the viability of sperm cells was observed. The leaf extract at a concentration of 200 μg mL-1 inhibited cell viability compared to the negative control (p< 0.001). The percentage of abnormal cells in leaf extract was shown in 100 and 200 μg mL-1, the conditions at which a higher percentage of morphological irregularities of observed (15% and 16% respectively). The results show that there was cellular detachment in the seminiferous epithelium in the experimental groups treated with methanolic extracts. Sperm death was observed without decreasing the number of sperm.

CONCLUSION

The methanolic extracts of Azadirachta indica have a modulating effect on the spermatogenesis of experimental rats through sperm morphological alterations.

摘要

背景

世界人口仍在增长，这对环境和发展中国家的经济增长产生了影响；因此，人们特别关注开发新的生育控制方法，重点是男性避孕。

目的

本研究旨在评估印楝叶和果甲醇提取物对长耳大仓鼠精子质量和睾丸组织学的影响。

方法

研究了印楝叶和果甲醇提取物对 24 只雄性大鼠的抗生育作用。将动物随机分为两组对照组和四组治疗组（每组 4 只）。分别给予叶和果提取物的浓度为 100 和 200μgmL-1。

结果

观察到精子细胞活力显著下降。与阴性对照组相比，浓度为 200μgmL-1 的叶提取物抑制了细胞活力（p<0.001）。在 100 和 200μgmL-1 的叶提取物中，观察到异常细胞的百分比更高（分别为 15%和 16%）。结果表明，在经甲醇提取物处理的实验组中，生精上皮细胞出现细胞脱落。精子死亡而精子数量没有减少。