Department of Exercise Physiology, Faculty of Physical Education & Sports Science, Kharazmi University, Tehran, Iran.
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
PLoS One. 2020 Aug 3;15(8):e0237148. doi: 10.1371/journal.pone.0237148. eCollection 2020.
The susceptibility to cardiovascular disease in offspring could be reduced prior to birth through maternal intervention, before and during pregnancy. We evaluated whether the initiation periods of maternal exercise in preconception and pregnancy periods induce beneficial effects in the adult male offspring. Thirty-two female rats were divided into control and exercise groups. The exercise groups involve exercise before pregnancy or the preconception periods, exercise during pregnancy, and exercise before and during pregnancy. The mothers in the exercise groups were run on the treadmill in different periods. Then the birth weight and weekly weight gain of male offspring were measured, and the blood and left ventricle tissue of samples were collected for analysis of the Sirtuin 6 (Sirt6) and insulin growth factor-2 (IGF-2) gene expression, serum levels of low-density lipoprotein (LDL), high-density lipoprotein (HDL), cholesterol (Cho), and triglycerides (TG). There was no significant difference in the birth weight of offspring groups (P = 0.246) while maternal HIIT only during pregnancy leads to reduce weekly weight gain of offspring. Our data showed that Sirt6 and IGF-2 gene expression was increased (P = 0.017) and decreased (P = 0.047) by maternal exercise prior to and during pregnancy, respectively. Also, the serum level of LDL (p = 0.002) and Cho (P = 0.007) were significantly decreased and maternal exercise leads to improves the running speed of the adult male offspring (p = 0.0176). This study suggests that maternal HIIT prior to and during pregnancy have positive intergenerational consequence in the health and physical readiness of offspring.
通过母亲在怀孕前和怀孕期间的干预，可以降低后代患心血管疾病的易感性。我们评估了母亲在孕前和孕期开始运动是否会对成年雄性后代产生有益影响。将 32 只雌性大鼠分为对照组和运动组。运动组包括在孕前或孕前期间运动、孕期运动以及孕前和孕期运动。运动组的母亲在不同时期在跑步机上跑步。然后测量雄性后代的出生体重和每周体重增加，并采集血液和左心室组织样本，分析 Sirtuin 6（Sirt6）和胰岛素生长因子-2（IGF-2）基因表达、血清中低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、胆固醇（Cho）和甘油三酯（TG）的水平。后代组的出生体重没有显著差异（P = 0.246），而仅在孕期进行 HIIT 会导致后代每周体重增加减少。我们的数据表明，Sirt6 和 IGF-2 基因表达分别通过母亲在孕前和孕期的运动而增加（P = 0.017）和减少（P = 0.047）。此外，血清 LDL（p = 0.002）和 Cho（p = 0.007）水平显著降低，母亲运动导致成年雄性后代的跑步速度提高（p = 0.0176）。这项研究表明，母亲在孕前和孕期进行 HIIT 对后代的健康和身体准备有积极的代际影响。
