Department of Kinesiology, East Carolina University, Greenville, NC 27834, USA.
Human Performance Laboratory, East Carolina University, Greenville, NC 27834, USA.
J Clin Endocrinol Metab. 2022 Jul 14;107(8):e3353-e3365. doi: 10.1210/clinem/dgac270.
Recent preclinical data suggest exercise during pregnancy can improve the metabolic phenotype not only of the mother, but of the developing offspring as well. However, investigations in human offspring are lacking.
To characterize the effect of maternal aerobic exercise on the metabolic phenotype of the offspring's mesenchymal stem cells (MSCs).
Randomized controlled trial.
Clinical research facility.
Healthy female adults between 18 and 35 years of age and ≤ 16 weeks' gestation.
Mothers were randomized into 1 of 2 groups: aerobic exercise (AE, n = 10) or nonexercise control (CTRL, n = 10). The AE group completed 150 minutes of weekly moderate-intensity exercise, according to American College of Sports Medicine guidelines, during pregnancy, whereas controls attended stretching sessions.
Following delivery, MSCs were isolated from the umbilical cord of the offspring and metabolic tracer and immunoblotting experiments were completed in the undifferentiated (D0) or myogenically differentiated (D21) state.
AE-MSCs at D0 had an elevated fold-change over basal in insulin-stimulated glycogen synthesis and reduced nonoxidized glucose metabolite (NOGM) production (P ≤ 0.05). At D21, AE-MSCs had a significant elevation in glucose partitioning toward oxidation (oxidation/NOGM ratio) compared with CTRL (P ≤ 0.05). Immunoblot analysis revealed elevated complex I expression in the AE-MSCs at D21 (P ≤ 0.05). Basal and palmitate-stimulated lipid metabolism was similar between groups at D0 and D21.
These data provide evidence of a programmed metabolic phenotype in human offspring with maternal AE during pregnancy.
最近的临床前数据表明，妊娠期间的运动不仅可以改善母亲的代谢表型，还可以改善发育中后代的代谢表型。然而，人类后代的研究还很缺乏。
描述母体有氧运动对后代间充质干细胞（MSCs）代谢表型的影响。
随机对照试验。
临床研究设施。
18 至 35 岁且妊娠≤16 周的健康成年女性。
母亲被随机分为 2 组：有氧运动（AE，n=10）或非运动对照组（CTRL，n=10）。AE 组按照美国运动医学学院的指南，在妊娠期间每周完成 150 分钟的中等强度运动，而对照组则参加伸展运动。
产后从后代的脐带中分离出 MSCs，并在未分化（D0）或肌生成分化（D21）状态下进行代谢示踪和免疫印迹实验。
D0 时的 AE-MSCs 胰岛素刺激的糖原合成的倍数变化高于基础水平，且非氧化葡萄糖代谢物（NOGM）的产生减少（P≤0.05）。在 D21 时，AE-MSCs 的葡萄糖向氧化（氧化/NOGM 比值）的分配显著升高，与 CTRL 相比差异有统计学意义（P≤0.05）。免疫印迹分析显示，AE-MSCs 在 D21 时的复合物 I 表达升高（P≤0.05）。在 D0 和 D21 时，两组的基础和棕榈酸刺激的脂质代谢相似。
这些数据为妊娠期间母体 AE 对人类后代的程序化代谢表型提供了证据。
