Jiao Yunfei, Li Justin, Xiang Junyi, Chen Zhengbo
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Dec 5;242:118706. doi: 10.1016/j.saa.2020.118706. Epub 2020 Jul 20.
We have developed a glucose oxidase (GOx)-mediated strategy for glucose detection, which is based on the intrinsic peroxidase-like activity of WS as a catalyst for the 3,3',5,5'-tetramethylbenzidine‑hydrogen peroxide (TMB-HO) reaction. The colorimetric assay involves two parts: generation of HO from the oxidation of glucose catalyzed by GOx, and WS nanosheets that catalyze the reaction between TMB and HO. In this colorimetric assay, the enhancement of colorimetric signals depends directly on the increased HO concentration, which, in turn, relies on the glucose concentration. The results show that the concentrations of the glucose were directly proportional to absorbance of the TMB solutions over a range of 1 nM-500 μM with a limit of detection of 0.1445 nM. In addition, this new colorimetric assay has been utilized for glucose detection in human serum with a satisfactory result.
我们开发了一种基于葡萄糖氧化酶（GOx）的葡萄糖检测策略，该策略基于WS作为3,3',5,5'-四甲基联苯胺-过氧化氢（TMB-H₂O₂）反应催化剂的内在过氧化物酶样活性。比色测定法包括两个部分：由GOx催化葡萄糖氧化生成H₂O₂，以及WS纳米片催化TMB与H₂O₂之间的反应。在这种比色测定法中，比色信号的增强直接取决于H₂O₂浓度的增加，而H₂O₂浓度又依赖于葡萄糖浓度。结果表明，在1 nM至500 μM的范围内，葡萄糖浓度与TMB溶液的吸光度成正比，检测限为0.1445 nM。此外，这种新的比色测定法已用于人血清中的葡萄糖检测，结果令人满意。
