College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, 266590, People's Republic of China.
Mikrochim Acta. 2021 Apr 23;188(5):171. doi: 10.1007/s00604-021-04819-9.
Urchin-like peroxidase mimics 5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin-functionalized CuCoO nanospheres (Por-CuCoO) has been fabricated as an excellent visual biosensor. X-ray diffractometry (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) have been employed to characterize the composition, morphologies, and elemental analysis of the as-synthesized Por-CuCoO. The catalytic activity of Por-CuCoO was evaluated by the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) with the aid of HO, which exhibited a visual blue change with an absorption maximum at 652 nm for only 10 s. The peroxidase-like behaviors of Por-CuCoO conformed to the Michaelis-Menten equation. Electrochemistry, radical scavenger, and fluorescence probe experiments verified that electron transfer, •O radicals, and holes (h) are the important factors during the catalytic oxidation of TMB. Based on the inhibition of dopamine (DA) on TMB oxidation, the Por-CuCoO-based colorimetric biosensor has been successfully constructed for sensitive determination of DA witha detection limit (LOD) of 0.94 μΜ. In addition, colorimetry was validated to detect DA in serum samples with high sensitivity and good selectivity. 5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin-functionalized urchin-like CuCoO (Por-CuCoO) with excellent peroxidase activity, ascribed to the synergistic effect between •O radicals and holes (h). A fast colorimetric sensor on the basis of Por-CuCoO has been constructed to quantitatively determine dopamine concentration in human serums.
具有 5,10,15,20-四(4-羧基苯基)卟啉功能化的刺猬状过氧化物酶模拟物 CuCoO 纳米球 (Por-CuCoO) 已被制备为一种出色的可视化生物传感器。X 射线衍射 (XRD)、扫描电子显微镜 (SEM) 和 X 射线光电子能谱 (XPS) 用于表征合成的 Por-CuCoO 的组成、形貌和元素分析。在 HO 的辅助下，通过显色基质 3,3',5,5'-四甲基联苯胺 (TMB) 评估了 Por-CuCoO 的催化活性，其仅在 10 秒内显示出具有最大吸收波长为 652nm 的视觉蓝色变化。Por-CuCoO 的过氧化物酶样行为符合米氏方程。电化学、自由基清除剂和荧光探针实验验证了电子转移、•O 自由基和空穴 (h) 是 TMB 催化氧化过程中的重要因素。基于多巴胺 (DA) 对 TMB 氧化的抑制作用，成功构建了基于 Por-CuCoO 的比色生物传感器，用于灵敏检测 DA，检测限 (LOD) 为 0.94 μΜ。此外，比色法已被验证可用于检测血清样品中的 DA，具有高灵敏度和良好的选择性。具有优异过氧化物酶活性的 5,10,15,20-四(4-羧基苯基)卟啉功能化刺猬状 CuCoO (Por-CuCoO)归因于•O 自由基和空穴 (h) 之间的协同作用。基于 Por-CuCoO 构建了一种快速比色传感器，可定量测定人血清中的多巴胺浓度。
