Zhang F, Zeng J, Li W W, Han Y K, Zhang P, Wang X S, Gu J P, Gu R J
The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang 453002, China.
Henan Key Lab of Biological Psychiatry, International Joint Research Laboratory for Psychiatry and Neuroscience of Henan, Xinxiang 453002, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020 Jul 20;38(7):485-489. doi: 10.3760/cma.j.cn121094-20190627-00262.
To investigate the association of single nucleotide polymorphism (rs3790088, rs4247109) with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) , and explore the influences of DEACMP genetic predisposition. From November 2006 to December 2017, 235 DEACMP cases and 429 acute carbon monoxide poisoning (ACMP) cases were selected. All ACMP patients were followed up for more than 90 days without DEACMP. The DNA in all blood samples were extracted with the blood Genome DNA Extraction Kit. The method of Sequenom Mass Array SNP technique was used to detect the genotype and allele of . All DEACMP patients were assessed every 3 days after hospitalization by the Hasegawa Dementia Scale (HDS) and Activity of Daily Living Scale (ADL) . The distribution of genotypes in conformty with Hardy-Weinderg law was analyzed by goodness-of-fit χ(2) test, and χ(2) test was used for association analysis. For rs3790088, there were 226 DEACMP cases and 414 ACMP cases. For rs4247109, there were 234 DEACMP cases and 428 ACMP cases. For rs3790088 and rs4247109 in gene: there were not significant differences in the gene genotype distribution and allele frequency of both DEACMP group and ACMP group (>0.05) . According to gender, there were not significant differences in gene genotype distribution and allele frequency between two female groups and two male groups (>0.05) . After analysis by genetic model, the genotype distributions in both DEACMP group and ACMP group were not significantly differences in three genetic models (codominant genetic model, recessive genetic model and dominant genetic model, >0.05) . It has not confirmed the genetic correlation between the two gene single nucleotide polymorphisms (rs3790088, rs4247109) of gene and the incidence of DEACMP.
探讨单核苷酸多态性（rs3790088、rs4247109）与急性一氧化碳中毒后迟发性脑病（DEACMP）的相关性，并探究DEACMP遗传易感性的影响。2006年11月至2017年12月，选取235例DEACMP患者和429例急性一氧化碳中毒（ACMP）患者。所有ACMP患者均随访90天以上且无DEACMP发生。采用血液基因组DNA提取试剂盒提取所有血样中的DNA。运用Sequenom Mass Array SNP技术检测基因型和等位基因。所有DEACMP患者住院后每3天采用长谷川痴呆量表（HDS）和日常生活活动量表（ADL）进行评估。采用拟合优度χ²检验分析基因型分布是否符合Hardy-Weinderg定律，并采用χ²检验进行关联性分析。对于rs3790088，有226例DEACMP患者和414例ACMP患者。对于rs4247109，有234例DEACMP患者和428例ACMP患者。对于该基因中的rs3790088和rs4247109：DEACMP组和ACMP组的基因基因型分布和等位基因频率均无显著差异（>0.05）。按性别分组，两组女性和两组男性的该基因基因型分布和等位基因频率均无显著差异（>0.05）。经遗传模型分析，DEACMP组和ACMP组在三种遗传模型（共显性遗传模型、隐性遗传模型和显性遗传模型）下的基因型分布均无显著差异（>0.05）。尚未证实该基因的两个单核苷酸多态性（rs3790088、rs4247109）与DEACMP发病率之间存在遗传相关性。
