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源自蛇毒液的假纳晶肽改变了表皮葡萄球菌细胞包膜的完整性，干扰了生物膜的形成。

Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis.

机构信息

Université de Rennes, CNRS, Institut de Génétique et Développement de Rennes (IGDR), UMR 6290, Rennes, France.

Laboratório de Biofilmes e Diversidade Microbiana, Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

出版信息

BMC Microbiol. 2020 Aug 3;20(1):237. doi: 10.1186/s12866-020-01921-5.

DOI:10.1186/s12866-020-01921-5
PMID:32746783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7397659/
Abstract

BACKGROUND

The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals against bacterial infections.

RESULTS

In this study we present an 11 amino acid-long peptide, named pseudonajide, which is derived from a Pseudonaja textilis venom peptide and has antimicrobial and antibiofilm activity against Staphylococcus epidermidis. Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Antibiofilm activity assays with pseudonajide concentrations ranging from 3.12 to 100 μM showed that the lowest concentration to inhibit biofilm formation was 25 μM. Microscopy analysis demonstrated that pseudonajide interacts with the bacterial cell envelope, disrupting the cell walls and membranes, leading to morphological defects in prokaryotes.

CONCLUSIONS

Our results suggest that pseudonajide's positives charges interact with negatively charged cell wall components of S. epidermidis, leading to cell damage and inhibiting biofilm formation.

摘要

背景

细菌耐药表型病例的增加是一个全球性的健康问题。科学界必须探索新的策略，以创造新的治疗选择。动物毒液是抗菌肽（AMPs）的良好来源，它们是开发新抗菌药物的理想候选物。来自蛇毒液的抗菌肽（CRAMPs）已被研究作为设计针对细菌感染的新型抗菌药物的模型。

结果

在这项研究中，我们提出了一种 11 个氨基酸长的肽，命名为 pseudonajide，它来源于一种 Pseudonaja textilis 毒液肽，对表皮葡萄球菌具有抗菌和抗生物膜活性。根据对各种具有抗菌活性的蛇毒液肽进行序列比对，选择了 pseudonajide。用浓度为 3.12 至 100 μM 的 pseudonajide 进行抗生物膜活性测定表明，抑制生物膜形成的最低浓度为 25 μM。显微镜分析表明，pseudonajide 与细菌细胞包膜相互作用，破坏细胞壁和细胞膜，导致原核生物形态缺陷。

结论

我们的结果表明，pseudonajide 的正电荷与表皮葡萄球菌的负电荷细胞壁成分相互作用，导致细胞损伤并抑制生物膜形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/919b0789109b/12866_2020_1921_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/4274706997f0/12866_2020_1921_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/c16b0f9cd005/12866_2020_1921_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/fde5f5c5d544/12866_2020_1921_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/71f738a0caf9/12866_2020_1921_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/00c6d4aa0161/12866_2020_1921_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/8d98a713b83e/12866_2020_1921_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/e685cd261230/12866_2020_1921_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/8d5aa7b3cde7/12866_2020_1921_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/27b8f34d0cb6/12866_2020_1921_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/544a7c5a489e/12866_2020_1921_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/919b0789109b/12866_2020_1921_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/4274706997f0/12866_2020_1921_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/c16b0f9cd005/12866_2020_1921_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/fde5f5c5d544/12866_2020_1921_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/71f738a0caf9/12866_2020_1921_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/00c6d4aa0161/12866_2020_1921_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/8d98a713b83e/12866_2020_1921_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/e685cd261230/12866_2020_1921_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/8d5aa7b3cde7/12866_2020_1921_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/27b8f34d0cb6/12866_2020_1921_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/544a7c5a489e/12866_2020_1921_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f693/7397659/919b0789109b/12866_2020_1921_Fig11_HTML.jpg

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