Department of Anatomy, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain; Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Tissue Cell. 2020 Aug;65:101366. doi: 10.1016/j.tice.2020.101366. Epub 2020 Apr 11.
Thyroid hormones are essential for metabolic rate regulation and play a role on the integrity of the salivary glands. Nigella sativa is a widely used plant in medicine. This study aimed to evaluate the effect of Nigella sativa oil (NSO) on the hypothyroidism-induced parotid gland pathological alterations. Rats were divided into four groups: control group, hypothyroid group: received daily oral carbimazole for 3 weeks, hypothyroid-NSO group: NSO was orally given for 4 weeks after hypothyroidism induction and NSO group: administrated NSO only for 4 weeks. After 7 weeks, all rats were sacrificed, serum thyroid hormones were estimated, and parotid glands were assessed by histopathological, immunohistochemical, ultrastructural and morphometric analyses. Hypothyroid group showed a significant decrease in thyroid hormones with increase in thyroid-stimulating hormone (TSH) levels and decrease in body and parotid weights compared to the control rats that were improved with NSO treatment. Sections of the hypothyroid group showed fibrosis, acinar cytoplasmic vacuolations, vascular congestion, ductal dilatation, wide intercellular canaliculi, nuclear pyknosis and decreased number of secretory granules. Also, there were decreased B-cell lymphoma 2 (Bcl-2) and increased p53, Bcl-2 Associated X (Bax) and alpha-smooth muscle actin (α-SMA) immune-expressions; with decreased Bax/ Bcl-2 ratio that all were attenuated by NSO. NSO ameliorates hypothyroidism-induced parotid changes by altering p53, Bax and Bcl-2 pathway.
甲状腺激素对代谢率的调节至关重要，并在唾液腺的完整性中发挥作用。黑种草是一种在医学中广泛使用的植物。本研究旨在评估黑种草油（NSO）对甲状腺功能减退引起的腮腺病理改变的影响。大鼠分为四组：对照组、甲状腺功能减退组：每天口服甲巯咪唑 3 周、甲状腺功能减退-NSO 组：甲状腺功能减退后口服 NSO 4 周、NSO 组：仅给予 NSO 4 周。7 周后，所有大鼠被处死，测定血清甲状腺激素，通过组织病理学、免疫组织化学、超微结构和形态计量学分析评估腮腺。与对照组相比，甲状腺功能减退组的甲状腺激素显著下降，促甲状腺激素（TSH）水平升高，体重和腮腺重量下降，用 NSO 治疗后得到改善。甲状腺功能减退组的切片显示纤维化、腺泡细胞质空泡化、血管充血、导管扩张、细胞间管腔增宽、核固缩和分泌颗粒减少。此外，B 细胞淋巴瘤 2（Bcl-2）减少，p53、Bcl-2 相关 X（Bax）和α-平滑肌肌动蛋白（α-SMA）免疫表达增加；Bax/Bcl-2 比值下降，这些均被 NSO 减弱。NSO 通过改变 p53、Bax 和 Bcl-2 途径改善甲状腺功能减退引起的腮腺变化。
