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一株 Cs-HK1 真菌来源的高分子量胞外多糖:超声降解、特性分析及体外粪便发酵。

A high-molecular weight exopolysaccharide from the Cs-HK1 fungus: Ultrasonic degradation, characterization and in vitro fecal fermentation.

机构信息

Food Safety and Technology Research Center, Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong; School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou, Guangdong, China.

Food Safety and Technology Research Center, Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

出版信息

Carbohydr Polym. 2020 Oct 15;246:116636. doi: 10.1016/j.carbpol.2020.116636. Epub 2020 Jun 13.

DOI:10.1016/j.carbpol.2020.116636
PMID:32747271
Abstract

This work was to examine the impact of power ultrasound (US) on the molecular properties of a high-molecular weight (MW) exopolysaccharide (EPS) from the Cs-HK1 medicinal fungus and the utilization, and prebiotic function of the US-treated EPS fractions in human fecal microflora in vitro. The US treatment caused notable reduction of intrinsic viscosity, average MW and aggregate size of EPS in water but no significant changes in the molecular structure. The US-treated EPS fractions were consumed more rapidly by the fecal microflora, resulting in a higher total level of short chain fatty acids. They also affected the relative abundance in the microflora more beneficially than the original EPS. The results suggest that power US is effective for modifying and improving the prebiotic properties of high-MW polysaccharides.

摘要

本研究旨在探讨功率超声(US)对 Cs-HK1 药用真菌高分子量(MW)胞外多糖(EPS)分子特性的影响,以及超声处理后的 EPS 级分在体外人粪便微生物群中的利用和益生元功能。超声处理导致 EPS 在水中的特性粘度、平均 MW 和聚集尺寸显著降低,但分子结构没有明显变化。粪便微生物群对超声处理后的 EPS 级分的消耗速度更快,导致短链脂肪酸的总水平更高。它们对微生物群的相对丰度的影响也比原始 EPS 更有益。结果表明,功率超声可有效用于修饰和改善高分子量多糖的益生元特性。

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