Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Int J Biol Macromol. 2020 Apr 15;149:1042-1050. doi: 10.1016/j.ijbiomac.2020.02.022. Epub 2020 Feb 5.
This study was to assess the anti-inflammatory potential of exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1. The EPS was isolated from the Cs-HK1 mycelial fermentation broth by ethanol precipitation and purified by deproteinization and dialysis. The EPS had a total sugar content of 74.8% and a maximum average molecular weight (MW) over 10 Da, and consisted mainly of glucose and mannose, and a small amount of galactose and ribose. In THP-1 and RAW264.7 cell cultures, EPS significantly inhibited lipopolysaccharide (LPS)-induced inflammatory responses of the cells including the release of NF-κB and several pro-inflammatory factors such as NO, TNF-α and IL-1β. In the murine model of LPS-induced acute intestinal injury, the oral administration of EPS to the animals effectively suppressed the expression of major inflammatory cytokines TNF-α, IL-1β, IL-10 and iNOS and alleviated the intestinal injury. The results suggest that the Cs-HK1 EPS has notable anti-inflammatory activity and can be a potential candidate for further development of new anti-septic therapeutics. To the best of our knowledge, this is the first report on the anti-inflammation of an EPS from C. sinensis fungal fermentation.
本研究旨在评估药用真菌蛹虫草（Cordyceps sinensis）Cs-HK1 所产生的胞外多糖（EPS）的抗炎潜力。该 EPS 是通过乙醇沉淀从 Cs-HK1 菌丝发酵液中分离出来的，并通过脱蛋白和透析进行纯化。EPS 的总糖含量为 74.8%，最大平均分子量（MW）超过 10 Da，主要由葡萄糖和甘露糖组成，还有少量的半乳糖和核糖。在 THP-1 和 RAW264.7 细胞培养中，EPS 显著抑制了 LPS 诱导的细胞炎症反应，包括 NF-κB 的释放和几种促炎因子，如 NO、TNF-α 和 IL-1β。在 LPS 诱导的急性肠道损伤的小鼠模型中，EPS 的口服给药有效地抑制了主要炎症细胞因子 TNF-α、IL-1β、IL-10 和 iNOS 的表达，并缓解了肠道损伤。结果表明，Cs-HK1 EPS 具有显著的抗炎活性，可能成为进一步开发新型抗感染治疗药物的候选物。据我们所知，这是首例关于蛹虫草真菌发酵的 EPS 抗炎作用的报告。
