Cold Spring Harb Protoc. 2020 Aug 3;2020(8):099705. doi: 10.1101/pdb.prot099705.
Cells for staining are usually prepared from one of three sources: adherent cells, suspension cells, or whole tissues. Antibodies generally are applied directly to the area of the cells or tissues that is being studied. The antibodies can be labeled directly or they can be detected by using a labeled secondary reagent that will bind specifically to the primary antibody. Detection reagents for cell staining can be labeled with fluorochromes, enzymes, gold, or iodine.
贴壁细胞、悬浮细胞或整个组织。抗体通常直接应用于正在研究的细胞或组织区域。抗体可以直接标记，也可以通过使用与初级抗体特异性结合的标记二级试剂来检测。细胞染色的检测试剂可以用荧光染料、酶、金或碘标记。
