Cold Spring Harb Protoc. 2020 Dec 1;2020(12):2020/12/pdb.prot099622. doi: 10.1101/pdb.prot099622.
Suspension cells can be prepared for staining by several different methods. A simple method for detecting intracellular antigens in cells that grow in suspension is to attach the cells to a solid substrate before fixation. This can be achieved by the use of a cytocentrifuge. For surface staining, suspension cells can be attached to slides by cross-linking with poly-l-lysine. Lysine can be polymerized to any desired length, and poly-l-lysine will bind to most solid supports through its charged side chains. The positively charged polymer will provide a site for binding of cells (which carry an overall negative charge). Although this cross-link is not covalent, it is sufficiently strong for most cell-staining techniques.
悬浮细胞可以通过几种不同的方法来制备用于染色。一种简单的方法是在固定之前将悬浮生长的细胞附着在固体基质上，以检测细胞内抗原。这可以通过使用细胞离心机来实现。对于表面染色，悬浮细胞可以通过与多聚赖氨酸交联附着到载玻片上。赖氨酸可以聚合到任何所需的长度，并且多聚赖氨酸将通过其带电侧链结合到大多数固体载体上。带正电荷的聚合物将为细胞（带整体负电荷）的结合提供位点。尽管这种交联不是共价的，但对于大多数细胞染色技术来说已经足够强了。
