Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
Infect Immun. 2020 Sep 18;88(10). doi: 10.1128/IAI.00470-20.
is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In this study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) that were comparable to the conditions in the native bronchus We used this ALI-PBECs model to study the infection and migration characteristics of Based on the results, we confirmed that was able to adhere to ALI-PBECs and disrupt mucociliary function. Importantly, could migrate to the basolateral chamber through the paracellular route but not the transcellular pathway, and this was achieved by reversibly disrupting tight junctions (TJs) and increasing the permeability and damaging the integrity of the epithelial barrier. We examined the migration ability of using an ALI-PBECs model for the first time. The disruption of the epithelial barrier allowed to migrate to the basolateral chamber through the paracellular route, which may be related to immune evasion, extrapulmonary dissemination, and persistent infection of .
是一种重要的猪呼吸道病原体，可引起持续性和继发性感染。然而，其发生的机制尚不清楚。在本研究中，我们建立了猪支气管上皮细胞（ALI-PBECs）的气液界面培养系统，该系统与天然支气管的条件相当。我们使用这个 ALI-PBECs 模型来研究 的感染和迁移特性。基于这些结果，我们证实 能够黏附于 ALI-PBECs 并破坏黏液纤毛功能。重要的是， 能够通过细胞旁途径而不是细胞内途径迁移到基底外侧室，这是通过可逆性破坏紧密连接（TJ）、增加通透性和破坏上皮屏障的完整性来实现的。我们首次使用 ALI-PBECs 模型研究了 的迁移能力。上皮屏障的破坏使得 能够通过细胞旁途径迁移到基底外侧室，这可能与免疫逃避、肺外传播和 的持续性感染有关。
