Veterinary Population Medicine, University of Minnesota, St Paul, Minnesota, USA
Veterinary Population Medicine, University of Minnesota, St Paul, Minnesota, USA.
Vet Rec. 2019 Oct 26;185(16):510. doi: 10.1136/vr.105475. Epub 2019 Aug 13.
Processing fluid (PF) is a sample type composed of fluids obtained from testicles and tails as the product of piglet processing. is a bacterium that colonises the respiratory tract of pigs and has rarely been detected in tissues outside the respiratory system. No data exist in the literature regarding detection of in PF or its use for herd monitoring of this pathogen. The main goal of this study was to evaluate the feasibility of detecting in PF. Testicles and tails of 21 conveniently selected litters from a commercial sow farm were collected, by litter, and tested for by real time-PCR. Daily aggregated processing tissues were collected for a two-month period to assess the detection of in PF. The comparison in the percentage of positive samples in fluids from testicles (38 per cent, 8/21) or tails (4.8 per cent, 1/21) was significantly different (P=0.023). The percentage of daily aggregated PF with cycle threshold values up to 37 was 52.9 per cent (9/17) and 26.7 per cent (4/15) for December and January, respectively. Overall, these data show detection of in PF for the first time and points at the potential use of this sample for monitoring of this bacterium in breeding farms.
加工液（PF）是一种由睾丸和尾巴中的液体组成的样本类型，是仔猪加工的产物。 是一种定植在猪呼吸道的细菌，很少在呼吸系统以外的组织中检测到。文献中没有关于 在 PF 中检测或其在该病原体的畜群监测中的应用的数据。本研究的主要目的是评估在 PF 中检测 的可行性。从一个商业母猪场中随机选择的 21 窝仔猪，每窝收集睾丸和尾巴，并通过实时 PCR 检测 。在两个月的时间里，每天收集聚合的加工组织，以评估 在 PF 中的检测情况。睾丸（38%，8/21）或尾巴（4.8%，1/21）中阳性样本的百分比差异有统计学意义（P=0.023）。12 月和 1 月的每日聚合 PF 中循环阈值值达到 37 的百分比分别为 52.9%（9/17）和 26.7%（4/15）。总体而言，这些数据首次表明 在 PF 中的检测，并指出该样本在监测养殖场中这种细菌的潜在用途。
