Sun Wen, Wang Haoyi
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China.
Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 100101, Beijing, China.
Gene Ther. 2020 Aug;27(7-8):312-320. doi: 10.1038/s41434-020-0181-5. Epub 2020 Aug 3.
Genome editing is a powerful tool, enabling scientists to alter DNA sequence at virtually any genome locus in any species. Different technologies have been developed employing programmable nucleases including meganuclease, zinc-finger nucleases, transcription activator-like effector nucleases, and most recently CRISPR-Cas systems. Chinese research groups are making important contributions at an increasing speed in genome editing field in recent years. In this review, we summarize recent progress made by Chinese scientists on the technological development of genome editing and beyond, focusing on the optimization and expanded application of existing genome editing tools, as well as the exploration of novel proteins as potential genome editing tools.
基因组编辑是一种强大的工具，使科学家能够在几乎任何物种的任何基因组位点改变DNA序列。已经开发出了不同的技术，这些技术采用了可编程核酸酶，包括归巢核酸内切酶、锌指核酸酶、转录激活样效应物核酸酶，以及最近的CRISPR-Cas系统。近年来，中国研究团队在基因组编辑领域正以越来越快的速度做出重要贡献。在这篇综述中，我们总结了中国科学家在基因组编辑技术发展及其他方面取得的最新进展，重点关注现有基因组编辑工具的优化和扩展应用，以及对作为潜在基因组编辑工具的新型蛋白质的探索。
