Department of Histology, Embryology, and Cytology, Tver' State Medical University, Ministry of Health of Russian Federation, Tver', Russia.
Federal Research Center for Nutrition and Biotechnologies, Moscow, Russia.
Bull Exp Biol Med. 2020 Jul;169(3):401-404. doi: 10.1007/s10517-020-04896-5. Epub 2020 Aug 3.
We studied the relationship between structural components of the wound (scab, leukocyte shaft, newly formed epithelium, and granulation tissue) and tissue components (epithelial and connective tissue) during spontaneous healing of skin wounds in rats. The regenerate structures were evaluated by microscopic morphometry. The number of vessels in 10 fields of view was calculated (at ×600), the dimensions of newly formed regenerate structures were determined: height of the scab, leukocyte shaft, granulation tissue, the border zone of the epithelium, and the length of the epithelial wedge. For the studied morphometric parameters, regression models of changes in the parameters of the regenerating wound were created depending on the term of the reparative process (days 7 and 14). The correlation analysis of the morphometric parameters of the regenerating wound revealed bilateral strong (direct and inverse) correlations in pairs. Most of the identified dependencies were linear and the changes in the number of vessels in the granulation tissue fit the quadratic regression law. The regularities observed by us during spontaneous healing of full-layer skin defects can be considered as control values for comparison with reparation of similar wounds under conditions of stimulation.
我们研究了大鼠皮肤伤口自然愈合过程中伤口（痂、白细胞轴、新形成的上皮和肉芽组织）和组织成分（上皮和结缔组织）的结构成分之间的关系。通过显微镜形态计量学评估再生结构。计算了 10 个视野中的血管数量（×600），确定了新形成的再生结构的尺寸：痂的高度、白细胞轴、肉芽组织、上皮边界区和上皮楔形的长度。对于研究的形态计量学参数，根据修复过程的期限（第 7 天和第 14 天），创建了再生伤口参数变化的回归模型。再生伤口形态计量学参数的相关分析显示出双边强（直接和间接）相关性。大多数确定的依赖性是线性的，肉芽组织中血管数量的变化符合二次回归规律。我们在全层皮肤缺损自然愈合过程中观察到的规律可以被认为是对照值，以便与刺激条件下类似伤口的修复进行比较。
