Qi Mengting, Zhu Xuerui, Yu Xiaoyi, Ai Min, Cai Weiwei, Du Bin, Hou Bao, Qiu Liying
Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China.
College of Pharmacy, Jiangnan University, Wuxi 214122, China.
Nanomaterials (Basel). 2021 Oct 25;11(11):2830. doi: 10.3390/nano11112830.
Chronic wound repair is a common complication in patients with diabetes mellitus, which causes a heavy burden on social medical resources and the economy. Hypaphorine (HYP) has good anti-inflammatory effect, and chitosan (CS) is used in the treatment of wounds because of its good antibacterial effect. The purpose of this research was to investigate the role and mechanism of HYP-nano-microspheres in the treatment of wounds for diabetic rats. The morphology of HYP-NPS was observed by transmission electron microscopy (TEM). RAW 264.7 macrophages were used to assess the bio-compatibility of HYP-NPS. A full-thickness dermal wound in a diabetic rat model was performed to evaluate the wound healing function of HYP-NPS. The results revealed that HYP-NPS nanoparticles were spherical with an average diameter of approximately 50 nm. The cell experiments hinted that HYP-NPS had the potential as a trauma material. The wound test in diabetic rats indicated that HYP-NPS fostered the healing of chronic wounds. The mechanism was through down-regulating the expression of pro-inflammatory cytokines IL-1β and TNF-α in the skin of the wound, and accelerating the transition of chronic wound from inflammation to tissue regeneration. These results indicate that HYP-NPS has a good application prospect in the treatment of chronic wounds.
慢性伤口修复是糖尿病患者常见的并发症，给社会医疗资源和经济带来沉重负担。羟基脯氨酸（HYP）具有良好的抗炎作用，壳聚糖（CS）因其良好的抗菌作用而用于伤口治疗。本研究的目的是探讨HYP纳米微球在糖尿病大鼠伤口治疗中的作用及机制。通过透射电子显微镜（TEM）观察HYP-NPS的形态。使用RAW 264.7巨噬细胞评估HYP-NPS的生物相容性。在糖尿病大鼠模型上制作全层皮肤伤口，以评估HYP-NPS的伤口愈合功能。结果显示，HYP-NPS纳米颗粒呈球形，平均直径约为50nm。细胞实验表明，HYP-NPS有作为创伤材料的潜力。糖尿病大鼠的伤口试验表明，HYP-NPS促进了慢性伤口的愈合。其机制是通过下调伤口皮肤中促炎细胞因子IL-1β和TNF-α的表达，加速慢性伤口从炎症向组织再生的转变。这些结果表明，HYP-NPS在慢性伤口治疗中具有良好的应用前景。
