Division of Histology and Microscopic Anatomy, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
Urol J. 2020 Jul 28;18(1):103-110. doi: 10.22037/uj.v16i7.5884.
In this study the role of nicotine (NCT) administration on the intensity of rat testicular tissue alterations induced by quinine (QU) was evaluated.
Forty adult Wistar rats were divided into four groups. Control (CON), NCT administrated (4 mg/kg) (NCT), QU treated (25 mg/kg for 7 days) (QU), and nicotine with quinine received (NCT+QU). After 28 days, serum testosterone and malondialdehyde (MDA) levels were measured. Testes and epididymides samples were prepared for determining tissue MDA levels, histomorphometry, microscopic indices of spermatogenesis, immunohistochemistry of p53 and sperm analysis.
Testosterone levels were decreased significantly (P = .0004) in treated groups compared to CON group. Serum MDA levels were increased significantly (P = .0004) in NCT and QU groups compared to CON group. Tissue MDA levels were increased significantly (P = .0012) in NCT+QU group in comparison to CON group. These parameters were changed significantly in NCT+QU group compared to QU group. Seminiferous tubules diameter decreased significantly (P < .0001) in treated groups compared to CON group and in NCT+QU group compared to QU group. The height of germinal epithelium decreased significantly (P = .0001) in NCT and NCT+QU groups compared to CON and QU groups. The number of Sertoli cells, spermatocytes, and spermatids decreased significantly in treated groups compared to CON group. The number of spermatogonia decreased significantly (P = .0017) in NCT and NCT+QU groups compared to CON group. The number of Sertoli cells, spermatogonia, and spermatocytes decreased significantly in NCT+QU group compared to QU group. All indices of spermatogenesis decreased in treated groups compared to CON group. The lowest mean of these indices was observed in NCT+QU group. The sperm viability decreased significantly (P < .0001) in treated groups compared to CON group. Sperm count and motility decreased significantly in NCT and NCT+QU groups compared to CON group. All experimental groups showed the over-expression of p53 compared to CON group.
The administration of nicotine could be involved in the exacerbation of testicular tissue alterations related to quinine therapy.
本研究评估了尼古丁（NCT）给药对奎宁（QU）诱导的大鼠睾丸组织改变强度的作用。
将 40 只成年 Wistar 大鼠分为四组。对照组（CON）、给予尼古丁（4mg/kg）的 NCT 组（NCT）、接受 QU 治疗（7 天 25mg/kg）的 QU 组和接受 NCT+QU 的尼古丁与奎宁组。28 天后，测量血清睾酮和丙二醛（MDA）水平。制备睾丸和附睾样本，以测定组织 MDA 水平、组织形态计量学、精子发生的显微镜指数、p53 的免疫组化和精子分析。
与 CON 组相比，治疗组的睾酮水平显著降低（P=0.0004）。与 CON 组相比，NCT 和 QU 组的血清 MDA 水平显著升高（P=0.0004）。与 CON 组相比，NCT+QU 组的组织 MDA 水平显著升高（P=0.0012）。与 QU 组相比，NCT+QU 组的这些参数发生了显著变化。与 CON 组相比，治疗组的精曲小管直径显著减小（P<0.0001），与 QU 组相比，NCT+QU 组的精曲小管直径显著减小。与 CON 和 QU 组相比，NCT 和 NCT+QU 组的生精上皮高度显著降低（P=0.0001）。与 CON 组相比，治疗组的 Sertoli 细胞、精母细胞和精子细胞数量显著减少。与 CON 组相比，NCT 和 NCT+QU 组的精原细胞数量显著减少（P=0.0017）。与 QU 组相比，NCT+QU 组的 Sertoli 细胞、精原细胞和精母细胞数量显著减少。与 CON 组相比，所有精子发生指数均降低，在 NCT+QU 组中最低。与 CON 组相比，治疗组的精子活力显著降低（P<0.0001）。NCT 和 NCT+QU 组的精子计数和活力均显著低于 CON 组。与 CON 组相比，所有实验组均显示 p53 过度表达。
尼古丁的给药可能参与了与奎宁治疗相关的睾丸组织改变的恶化。
