Osinubi A A, Daramola A O, Noronha C C, Okanlawon A O, Ashiru O A
Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria.
West Afr J Med. 2007 Jul-Sep;26(3):217-21. doi: 10.4314/wajm.v26i3.28313.
We have previously demonstrated that quinine is a testicular toxicant in Sprague-Dawley rat.
To describe the changes in the testicular levels of testosterone and lipid peroxidation secondary to quinine and ascorbic acid administration in rats.
Twenty male Sprague-Dawley rats per group were assigned to one of three treatment groups: 0 mg quinine and 0 mg ascorbic acid/kg body weight (control); 10 mg quinine/ kg BW; and 10 mg quinine plus 0.1 mg ascorbic acid/kg BW. Rats were intramuscularly administered their respective doses of quinine five days in a week and ascorbic acid three days in a week for eight weeks. All the animals were sacrificed at the end by decapitation. Seminal analysis was performed on tubular fluid from caudal epididymides. Evaluations were made for testicular levels of testosterone and lipid peroxidation through malondialdehyde (MDA). Testicular specimens were also processed for histology under light microscopy.
Quinine significantly (p < 0.01) increased free radicals (from elevation of MDA) and decreased testosterone in the testis compared with those of the control group and those treated with a combination of quinine and ascorbic acid. The semen of rats treated with only quinine demonstrated a significantly (p < 0.001) lower sperm concentration and motility compared to the controls and those treated with quinine plus ascorbic acid. Microscopic examination of cross-sections of seminiferous tubules also showed that ascorbic acid partially protected against quinine -induced testicular effects.
Ascorbic acid has beneficial effect and protects against quinine-induced testicular reduction of testosterone.
我们之前已证明奎宁对斯普拉格-道利大鼠具有睾丸毒性。
描述大鼠给予奎宁和抗坏血酸后睾丸中睾酮水平及脂质过氧化的变化。
每组20只雄性斯普拉格-道利大鼠被分配至三个治疗组之一：0毫克奎宁和0毫克抗坏血酸/千克体重（对照组）；10毫克奎宁/千克体重；以及10毫克奎宁加0.1毫克抗坏血酸/千克体重。大鼠每周五天肌肉注射各自剂量的奎宁，每周三天注射抗坏血酸，持续八周。所有动物在实验结束时断头处死。对来自附睾尾部的管状液进行精液分析。通过丙二醛（MDA）评估睾丸中睾酮水平和脂质过氧化情况。睾丸标本也进行处理以用于光学显微镜下的组织学检查。
与对照组及接受奎宁和抗坏血酸联合治疗的组相比，奎宁显著（p < 0.01）增加了睾丸中的自由基（因MDA升高）并降低了睾酮水平。仅接受奎宁治疗的大鼠精液与对照组及接受奎宁加抗坏血酸治疗的大鼠相比，精子浓度和活力显著（p < 0.001）降低。对生精小管横截面的显微镜检查还表明，抗坏血酸可部分预防奎宁诱导的睾丸效应。
抗坏血酸具有有益作用，可预防奎宁诱导的睾丸睾酮水平降低。
