Xu Jiasu, Zhang Ya, Su Xiaosong, Zhang Shiyin, Ge Shengxiang
School of Public Health, Xiamen University, Xiamen 361102, Fujian, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jul 25;36(7):1283-1292. doi: 10.13345/j.cjb.190518.
Point-of-care testing (POCT) is a test method performed on the sampling site or patient bedside. Accurate results can be achieved rapidly by the application of portable analytical instruments and compatible reagents. It has been widely used in the field of in vitro diagnosis (IVD). Paper-based microfluidics technology has great potential in developing POCT due to its advantages in low cost, simple operation, rapid detection, portable equipment, and unrestricted application conditions. In recent years, the development of paper-based microfluidic technology and its integration with various new technologies and methods have promoted the substantial development of POCT technology and methods. The classification and characteristic of the paper are summarized in this review. Paper-based microfluidic sample pretreatment methods, the flow control in the process of reaction and the signal detecting and analyzing methods for the testing results are introduced. The research progress of various kinds of microfluidic paper-based analytical devices (μPADs) toward POCT in recent years is reviewed. Finally, remaining problems and the future prospects in POCT application of paper-based microfluidics are discussed.
即时检验（POCT）是一种在采样现场或患者床边进行的检测方法。通过应用便携式分析仪器和兼容试剂，可以快速获得准确结果。它已在体外诊断（IVD）领域得到广泛应用。基于纸的微流控技术因其成本低、操作简单、检测快速、设备便携以及应用条件不受限制等优点，在即时检验的发展中具有巨大潜力。近年来，基于纸的微流控技术的发展及其与各种新技术和方法的整合推动了即时检验技术和方法的实质性发展。本文综述了纸的分类和特性。介绍了基于纸的微流控样品预处理方法、反应过程中的流动控制以及检测结果的信号检测和分析方法。综述了近年来各种基于微流控纸的分析装置（μPADs）在即时检验方面的研究进展。最后，讨论了基于纸的微流控技术在即时检验应用中存在的问题和未来前景。
