Sun Xue, Dong Yonghua, Wang Na, Cui Wenhui, Liao Xianyan, Liu Li
School of Life Sciences, Shanghai University, Shanghai 200444, China.
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jul 25;36(7):1356-1364. doi: 10.13345/j.cjb.190519.
Salinity is the most important factor for the growth of crops. It is an effective method to alleviate the toxic effect caused by salt stress using saline-alkali-tolerant and growth-promoting bacteria in agriculture. Seven salt-tolerant bacteria were screened from saline-alkali soil, and the abilities of EPS production, alkalinity reduction and IAA production of the selected strains were investigated. A dominant strain DB01 was evaluated. The abilities of EPS production, alkalinity reduction and IAA production of strain DB01 were 0.21 g/g, 8.7% and 8.97 mg/L, respectively. The isolate was identified as Halomonas aquamarina by partial sequencing analysis of its 16S rRNA genes, and had the ability to inhibit the growth of Fusarium oxysporum f. sp., Alternaria solani, Phytophthora sojae and Rhizoctonia cerealis. It also could promote root length and germination rate of wheat seedlings under salt stress. Halomonas aquamarina can provide theoretical basis for the development of soil microbial resources and the application in saline-alkali soil improvement.
盐分是影响作物生长的最重要因素。在农业中使用耐盐碱且促进生长的细菌来减轻盐胁迫造成的毒性影响是一种有效方法。从盐碱土中筛选出7株耐盐细菌，并对所选菌株的胞外多糖产生能力、碱度降低能力和吲哚乙酸产生能力进行了研究。对一株优势菌株DB01进行了评估。菌株DB01的胞外多糖产生能力、碱度降低能力和吲哚乙酸产生能力分别为0.21 g/g、8.7%和8.97 mg/L。通过对其16S rRNA基因进行部分测序分析，该分离株被鉴定为海蓝盐单胞菌，具有抑制尖孢镰刀菌、番茄链格孢、大豆疫霉和禾谷丝核菌生长的能力。它还能在盐胁迫下促进小麦幼苗的根长和发芽率。海蓝盐单胞菌可为土壤微生物资源的开发及其在盐碱土改良中的应用提供理论依据。
