Chemical, Pharmacological and Galenic Development Laboratory, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
Department of Chemistry, Faculty of Science, Northern Border University, Arar, Saudi Arabia.
Nat Prod Res. 2021 Dec;35(24):5888-5893. doi: 10.1080/14786419.2020.1800697. Epub 2020 Aug 4.
In this study, Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC values of 26.9 and 11.7 µg/mL, respectively.
在这项研究中，从突尼斯西北部的两个不同地点采集了 Poir.植物材料，并通过水蒸馏法提取了其挥发油（EO）。气相色谱-质谱联用（GC-MS）和火焰离子化检测（GC-FID）用于挥发性成分的定性分析和定量。百里酚（50.1-52.8%）被鉴定为两种 EO 的主要成分。为了评估 EO 作为抗真菌剂的潜在应用，测试了它们对六种真菌菌株的抑制作用；一个样品表现出很强的抗真菌活性（MIC = 40-400 μ g/mL）。使用比色 MTT 法，在两种人癌细胞系，即结肠（HCT116）和乳腺腺癌（MCF7）上研究了其抗增殖活性。同样，同一个样品对两种癌细胞系均表现出良好的抗增殖活性，IC 值分别为 26.9 和 11.7 μ g/mL。
