CRISPR/Cas：动物模型中基因组编辑的成功工具。
CRISPR/Cas: A Successful Tool for Genome Editing in Animal Models.
机构信息
School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
出版信息
Crit Rev Eukaryot Gene Expr. 2020;30(3):239-243. doi: 10.1615/CritRevEukaryotGeneExpr.2020028791.
CRISPR/Cas9 is an innovative molecular tool that is utilized in advanced biological applications. This review focuses on modifying the genomes of a wide range of animals by CRISPR/Cas9 for greater usability and higher efficiency, providing an overview of the function and mechanism of this system and the utilization of this system for medicinal research. The type II CRISPR-Cas system is found in the Enterobacteriaceae bacteria family, which uses this system as a defense against invading phages and plasmids. This system can be engineered to direct its action toward a targeted site for the modification of a specific genome.
CRISPR/Cas9 是一种创新的分子工具，广泛应用于先进的生物学研究中。本文主要综述了 CRISPR/Cas9 在改良多种动物基因组方面的应用，旨在提高其可用性和效率。文中概述了该系统的功能和作用机制，以及其在医学研究中的应用。Ⅱ型 CRISPR-Cas 系统发现于肠杆菌科细菌中，该系统可用于防御噬菌体和质粒的入侵。该系统可被设计靶向特定的基因组进行修饰。
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